The Construction Of Electrochemical Sensor Based On Various Nanomaterials And Its Application In Food And Bioanalysis

Electrochemical sensors and immunosensing technology based on nanomaterials,have the advantages of good selectivity,high sensitivity,fast and efficient,simple operation and low cost.They are widely used in food and bioanalysis.In this paper,NiCo2O4/polyethyleneimine(PEI)nanocomposites was used to constructe the electrochemical sensors for 6-benzylaminoadenine(6-BAP)detection of bean sprouts;A sandwich immunosensor based on carboxylazed ionic liquid/helical carbon nanotubes(CIL-HCNTs)nanocomposites was constructed for cancer markers troponin I(cTnI)detection of serum;And amino polyisophilic liquid/ordered mesoporous carbon(CMK-3/PIL-NH2)nanocomposites was used to constructe the electrochemical sensors for the detection of ovarian cancer markers(CA125),which provides a new strategy for the development and application of electrical sensors.In the first chapter,a kind nanosuperstructure NiCo2O4 nanocomposites was prepared by simple hydrothermal method.The NiCo2O4/PEI nanocomposites have excellent conductivity and catalytic activity.A new electrochemical platform for sensitive determination of 6-BAP was designed based on NiCo2O4/PEI nanocomposites.In the second chapter,CIL-HCNTs nanocomposites were prepared by simple ultrasonic one-step method.Moreover,Ab2-Fc-COOH was used as a signal probe to construct a highly selective and sensitive sandwich type immunosensor for cTnI detection based on CIL-HCNTs.In the third chapter,a new type of CMK-3/PIL-NH2 nanocomposite was obtained by free radical polymerization.At the same time,the antibody biomarkers labeled with horseradish peroxidase(HRP)and HCNTs as the signal-amplification probe,which was used to construct the competitive immunosensor based on CMK-3/PIL-NH2 nanocomposite for CA125 detection.These new constructed sensors have high sensitivity and stability,wihch could be effectively used in food and bioanalysis.