The Study Of Biotin Pectin Polysaccharide Nanoparticles As Drug Carrier

Pectin polysaccharide has good biocompatibility,it has a wide range of sources and is safe and non-toxic.It can be used as an anti-tumor drug carrier in the treatment of cancer.In recent years,researchers have produced a lot of pectin polysaccharide drug carrier,but there are still some shortcomings,such as poor targeting selectivity to tumor cells and unreasonable scale range of pectin polysaccharide particles(many of which are micron scale).In order to improve the target selectivity of pectin polysaccharide and reduce the size of pectin polysaccharide particles,so that the pectin polysaccharide particles play a greater role as a drug carrier.In this paper,the biotin target was modified on the pectin polysaccharide molecule,the active target drug carrier material was designed and synthesized.Biotinylated pectin polysaccharide nanoparticles were prepared by the cross-linking of calcium ions and emulsified ultrasonic method.By investigating the effect of preparation conditions on the size of nanoparticles,the optimal preparation method was investigated.By investigating the drug release behavior of drug loaded nanoparticles in vitro,the controlled-release properties of polysaccharide nanoparticles were studied.The main contents of this paper are as follows:Biotinylated pectin polysaccharide(Bio-PEC)was obtained by grafting biotin on the free hydroxyl group of pectin polysaccharide(PEC)molecular chain by esterification.The synthesized products were characterized by Fourier transform infrared(FT-IR)and X ray powder diffraction(XRD).The degree of substitution of biotin was determined by elemental analyzer.The results showed that biotin was successfully modified to the surface of pectin molecules through the ester bond,and the degree of substitution of biotin in the synthetic product was 37%.According to the properties of pectin polysaccharide,a suitable and feasible method was selected to prepare nanoparticles,that is,Bio-PEC nanoparticles were prepared by emulsionultrasonic method under the action of calcium ions.The morphology,size and surface charge of the nanoparticles were characterized by scanning electron microscopy,Zeta potential and particle size analyzer.The results show that the Bio-PEC nanoparticles are approximately spherical and have uniform distribution.There is no obvious agglomeration,the size of the particle size is about 200 nm and the surface charge is about-30 mV.Doxorubicin(DOX)was used as a model drug,and Bio-PEC drug-loaded nanoparticles were prepared by electrostatic adsorption.The surface charge and particle size distribution of the nanoparticles were investigated by Zeta potential and particle size analyzer.The incorporation of doxorubicin in drug-loaded nanoparticles was observed by inverted fluorescence microscope.The drug release of drug loaded nanoparticles in PBS solution at different pH was determined by UV spectrophotometry.The results show that the prepared biotinylated pectin loaded adriamycin(DOX/Bio-PEC)has a particle size of about 323 nm,and the surface charge is about-17.45 mV.It can be seen from the drug release curve of drug-loaded nanoparticles that when the pH is around 6.8,the cumulative release of drugs is high and the drug carrier particles have a significant slow release effect on DOX.