Research To Separate The Cholesterol In Animal Fat

Cholesterol is an indispensable part of the human body,which has many benefits to use,but high level cholesterol in blood is also the most important risk factors of atherosclerosis.So it is imperative to study the food which has low level cholesterol.Molecular imprinting technique is becoming increasingly recognized as a powerful technique of preparing synthetic polymers,the magnetic molecularly imprinted polymer core/shell nanoparticles has two advantages of super paramagnetic and high selectivity.In this study,a new molecular imprinting polymer was synthesized for Fe₃O₄ magnetic nanoparticles coated cholesterol-imprinted polymer with controlled size.Cholesterol template with allyl-?-cyclodextrin as monomers was used to form novel hydrophilic MIPs in aqueous media by the interaction of lipophilic group in cholesterol and the hydrophilic group in the?-cyclodextrin.The cholesterol-binding properties of the obtained polymers were analyzed by GC.FT-IR spectroscopy was use to confirm the structure of magnetic MIPs,which verified the successful introduction of the functional groups in the imprinted cavities.The resulting cholesterol-imprinted polymer coating Fe₃O₄ magnetic nanoparticles exhibit a much higher specific recognition and saturation magnetization.The imprinted polymer was evaluated by static and kinetic adsorption experiments.The results show that the imprinted polymer offered a faster kinetic for the adsorption and desorption of the template cholesterol.To evaluate the usefulness of the resulting products,the hybrid nanoparticles have been used for biochemical separation of cholesterol in animal fat or animal product.The average cholesterol removal of different animal fat samples were from 83.13%to92.70%.The limit of detection?LOD?of the detective method of cholesterol is0.24mg/100g.