Research On The Determination Of Gastrodin And Total Gastrodin In Health Proucts

Gastrodia has sedative analgesia,lower blood pressure,liver damage treatment,enhance immunity,and anti-aging effects.The main component of Gastrodia elata is gastrodin,which is about 0.33%⁰.67%in Gastrodia.Therefore,the size of gastrodin content is a reflection of the quality of the indicators of gastrodin.In recent years,with the progress of society,economic development and improvement of people's living standards,with the social progress and economic development,the global residents of health consumption is rising year by year,the demand for nutrition and health care products or drugs is very strong.Therefore,the demand for the object,relying solely on a single herb to get the health effect of gastrodin is far from enough,therefore,more and more Gastrodia products have been developed to meet the needs of society.And now on the extraction and detection of gastrodin,there is no existing implementation of the national standard method,only in the"Chinese Pharmacopoeia"Tianma under the extraction and detection of gastrodin,and"Chinese Pharmacopoeia"on the detection of gastrodin test only free The content of gastrodin,the study found that the combination of gastrodin-gastrodin and citric acid condensation of ester components,such as balicin,balicillin B,balicin C far higher than the"Chinese Pharmacopoeia"Gastrodia tested in the content of free gastrodin,and there is no method for the extraction and determination of total gastrodin,resulting in a number of Gastrodia products with reference to"Chinese Pharmacopoeia"method of detection of gastrodin content is only detected in the sample of free gastrodin,No detection of total gastrodin,which can not be an objective and objective response to the evaluation of Tianma products,so as to fully control the quality of Gastrodia products.?1?This study estabilish the HPLC-DAD method.The mobile phase is acetonitrile:0.05%phosphoric acid solution?3:97?;and the column temperature is 30?;injection volume is10?L;flow rate is 1 mL/min.?2?Establishing the methods of free gastrodin in various types of capsules,powder,liquid health products The free gastrodin was extracted with 70%ethanol solution,the extraction temperature was 70?,and the extraction time was 50 min.The free gastrodin was extracted from the sample and allowed to cool to 50.00m L volumetric flask.10.00 m L of the filtrate was collected and concentrated to near dryness.The residue was dissolved in the mobile phase and transferred to a 25.00m L volumetric flask.The volume was adjusted to a scale with a mobile phase.After shaking,it was filtered through a 0.45?m filter.Using HPLC-DAD to dected the content of free gastrodin in the sample solution.The linear ranger of gastrodin was5.00?g·m L^-1400.0?g·m L^-1 with square of the correlation coefficient above 0.9998.The average recoveries of gastrodin were 94.0%¹00%and the relative standard deviations?RSD?of the samples were in the range of 0.30%⁰.90%.The limit of detection was0.5mg/100g,the limit of detection was 2mg/100g.?3?Take 10mL supernatant of all kinds of samples,filter out,add 2.0%sodium hydroxide solution,transfer to 10mL volumetric flask,dilute with 2.0%sodium hydroxide solution,shake.Precision drawing 2.00 m L of the solution,placed in a 10mL volumetric flask,hydrolysis at a temperature of 2h,and then adjusted to pH 3.0mol·L^-1 hydrochloric acid 3 to4,dilute with mobile phase,shake,that was the total gastrodin sample solution.After shaking,it was filtered through a 0.45?m filter.Using HPLC-DAD to dected the content of t gastrodin in the sample solutions.The linear ranger of gastrodin was5.00?g·m L^-1400.0?g·m L^-1 with square of the correlation coefficient above 0.9998.The average recoveries of gastrodin were 94.0%¹00%and the relative standard deviations?RSD?of the samples were in the range of 0.30%⁰.90%.The limit of detection was0.5mg/100g,the limit of detection was 2mg/100g.Experiments show that the ultrasonic extraction method is feasible to extract free gastrodin in Tianma health products.Compared with the method of"Chinese Pharmacopoeia",the ultrasonic extraction method is simple,the controllable is stronger,the experiment time is short,The experimental efficiency,greatly improved is the number of batch samples,in line with the requirements of commercial laboratories.At the same time,the extraction and determination of total gastrodin in the sample can be establishedAs a commercial laboratory,it can provide customers with more data to control the product development and production.