The Construction And Biological Evaluation Of Hyaluronic Acid Coating With Releasing NO

Inhibition of restenosis and late thrombosis is a major end-point problem for vascular stent modification in treating atherosclerotic.The mainstream academic community generally believes that rapid endothelialization on the surface of implanted materials may effectively reduce the risk of late thrombosis and restenosis.The biocompatibility of cardiovascular implant materials is improved by immobilizing biomolecules,this mature surface modification method has been well studied and applied within a certain range.In this paper,100 Kda molecular weights of hyaluronic acid and selenocystamine have been grafted onto the surface of 316 L stainless steel coated with dopamine and hexamethylenediamine blended films by the amide reaction,in order to construct a dualistic bioinspired endothelial multi-functional coating.To achieve anticoagulation,anti-proliferative and anti-inflammatory,at the same time accelerate repair and regeneration of blood vessels on the surface of the material.The main content of the paper is as follows:In this paper,the samples were characterized by a series of material characterization methods,such as water contact angle(WCA),surface hyaluronic acid quantification,Fourier transform infrared spectrometer(FTIR),photoelectron spectroscopy(XPS),atomic force microscopy(AFM),and nitric oxide(NO)releasing efficiency and stability.The results of WCA,FTIR and XPS indicated that HA and selenocystamine are successively fixed on the surface of the sample,the HA@NO biomimetic endothelial multi-functional coating was successfully prepared.The results of platelet adhesion and activation,semi-in vivo whole blood test,endothelial cell static culture,smooth muscle cell static culture,and macrophage static culture experiment in vitro showed that the binary(HA@NO)biomimetic endothelial multi-functional coating reduce the amount of platelet adhesion and activation,inhibit smooth muscle proliferation and show a good anti-inflammatory response on the surface of the material by multiple pathways of synergizing or complementing,while promoting endothelial cell proliferation and release of functional factors(prostacyclin),showed excellent blood and cytocompatibility.The experimental results of abdominal aorta implantation in SD rats showed thahe binary(HA@NO)biomimetic endothelial multi-functional coating not only inhibited the formation of tissue hyperplasia and inflammation,but also significantly promoted intimal repair and regeneration,showed excellent histocompatibility.In summary,the extracellular matrix(hyaluronic acid,HA)and cell signaling factor(nitric oxide,NO)was selected as a functional molecule,and it was immobilized on the surface of 316 L stainless steel with dopamine and hexamethylenediamine blend film,investigated whether the binary bionic endothelial coating constructed by the medium molecular weight of hyaluronic acid and physiological release concentration of nitric oxide,has better performance of inhibiting platelet adhesion and activation,smooth muscle proliferation and good anti-inflammatory response,and that realizing to rapidly promoting the reconstruction and repair of endothelial cell.