Fluorescent Probe With 1,8-naphthalene Diamine As Reactive Group For ROS/RNS

There are many fluorescent probes for detecting nitric oxide and hypochlorous acid.The fluorescent probe consists of three parts,namely,a fluorophore,linking groups,and a recognition group.Generally,the involved fluorophores include BODIPY,rhodamine,fluorescein,coumarin,metal complexes and others.For nitric oxide fluorescent probes,the reported NO detection groups contains o-phenylenediamine,five-membered cyclic amides,secondary amines,and others.Amongst them,o-phenylenediamine is the most widely used as NO reactive group.Recognition group of HOCl and ClO-of fluorescent probes involved such as fluorene,1,8-naphthalene diamine,1,3-oxathiolane,ferrocene,formamide,1,4-thiazepine,and so on.However,most of above mentioned probes only response to one species of HOCl or NO.However,both HOCl and NO are involved in various diseases such as atherosclerosis,cardiovascular diseases and tumors.It is thus essential to develop new fluorescent probe to discriminate HOCl from NO with different signal responses,which could facilitate the deep understanding of the corresponding psychological and pathological process.Here we report a new fluorescent probe(QBN)of HOCl and NO with dual-channel,which is featured by the investigation of utilizing 1,8-naphthalene diamine as a recognition group.The major findings of the studies can be summarized as follows.Structure of QBN contains 6-phenylethylyl-quinoline as fluorophore and 1,8-naphthalene diamine as reactive group.Reaction of NO with QBN was proposed as nitrosation of the free amine group while HOCl can oxidize the A ring of naphthalene to quinine analog inspecificity.Thereof a dual-channel emission is fulfilled due to the two different fluorescent responses.