Effects Of Micro-pollutants On Microbial Community And Pathogenic Bacteria In Drinking Water Network

Water quality in drinking water distribution systems(DWDSs)has a major role to play in ensuring the safety of drinking water and it is affected by many factors such as disinfection by-products,pipe corrosion and microbial regrowth.Different water treatment processes would result in great contrast in water quality in drinking water treatment plant(DWTP),and finally lead to significant shift of that in DWDSs.The aim of this study was to examine the effects of micro-pollutants on bacterial regrowth in drinking water pipelines.The main conclusions were as follows:(1)Effects of micro-pollutants on opportunistic pathogens in drinking water pipelines.The sand filter in water plant water to add sulfadiazine and ciprofloxacin,the method of quantitative PCR and the different pipe network water microorganism secretion of extracellular polymers(EPS and resistant genes were analyzed,and studied the different microscopic contaminants on the pipe network water microorganisms including pathogenic bacteria influence of regeneration.Found that sulfadiazine and ciprofloxacin join network out of total bacteria in water,birds,mycobacteria,spines amoebas and hartz increased levels of insect.In addition,after micro pollutants into the pipe network of drug-resistant microbes secrete more extracellular polymers(EPS,thus easier to grow on particulate matter,so network larger quantity increased,the particle size significantly enhanced with particles adhesion microbes including conditional pathogenic bacteria resistance to chlorine,therefore with particulate adhesion microbes including conditional pathogenic bacteria increased obviously.In addition,these microbes increased more obviously under the condition of compound pollution.(2)Effects of micro-pollutants on microbial communities in drinking water pipelines.By sequencing pyrophosphate(454 sequencing)technology and quantitative polymerase chain reaction(PCR)analysis of different network microbial species composition and quantity.The results show that the network biofilm microbial community structure and water has obvious difference,added antibiotics of different microbial communities exist the phenomenon of species succession.In addition,found sulfadiazine and ciprofloxacin,obviously reduces the network freely suspended in water and particles adhesion of microbial community diversity.But,out of the water freely suspended in the microbial community,increased levels of bdellovibrio and particle adhesion of raw silk micro bacteria of the genus content in the microbial community.Biofilm is also in the raw silk micro bacteria content increase.Compound of the presence of micropollutants,these microbes including more obvious with the increase of the pathogenic bacteria.(3)Effects of micro-pollutants on bacterial regrowth on the biofilm of the drinking water pipelines.The sand filter in water plant water adding method of sulfadiazine and ciprofloxacin,studied the different micro pollutants influence of pathogenic bacteria in biofilm regeneration.First through quantitative PCR analysis found that the addition of sulfadiazine and ciprofloxacin ciprofloxacin slightly reduces the network out of pseudomonas aeruginosa in water,the content of spines amoebas,but pseudomonas aeruginosa,acanthamoeba amoeba in sulfadiazine and compound pollution network content is increased slightly.Overall,the antibiotic impact on conditional pathogenic bacteria biofilm is not very big.But,as a result of enzyme and EPS makes on biofilm microbial growth,microbial amount increased.In addition,sulfadiazine and ciprofloxacin increased pipeline of the water content of microbial resistance genes,especially under the condition of compound pollution resistance genes increase the highest.