Studies On Antifungal Active Component Of Phyllostachy Pubescens Extract

In this paper,the antifungal activities of extract from Phyllostachys pubescens against Gibberella sanbinetti were tested using bioassay methods;Guided with the method of bioactivity tracking,the antifungal activities components were isolated,purified and identified basing on the previous work.The main results were summarized as following:1.The antifungal activities of Phyllostachys pubescens extract by 95% ethanol against Gibberella sanbinetti was examined.The results showed that extract of Phyllostachys pubescens has antifungal activities on the selected pathogens;the inhibition rates of Phyllostachys pubescens extract to ibberella sanbinetti ?Colletotrichum gloeosporioides?Phytophthoracapsici after 48 h were 100%?75.12%?60.66%,respectively.The inhibition rates of Physalospora piricpla Nose?Fusicladium plrinum?Botrytis cinerea P were less than 50%,completely.The value of EC50 of Phyllostachys pubescens to Gibberella sanbinetti was 25.2m g/L(y=19.490x+0.8403,R2=0.9926).2.The antifungal activities of petroleum extract and ethyl extract against Gibberella sanbinetti were examined,which were extracted from the extraction of Phyllostachys pubescens.The results showed that,the inhibition rates of ethyl fraction and petroleum fraction to Gibberella sanbinetti after 48 h were 100% and35.74%,respectively.Therefore the antifungal activity of ethyl extract from Phyllostachys pubescens was stronger than the extract of petroleum.The value of EC50 of ethyl extract to Gibberella sanbinetti was 18.4mg/L(y=17.498x+17.794,R2=0.9359).3.The antifungal activities of the 8 fractions against Gibberella sanbinetti were examined,which were collected from the ethyl extract of Phyllostachys pubescens by column chromatography.The bioassay suggested that the first(K1)?the second(K2)?the third(K3)and the forth(K4)fraction had strong antifungal activities,while other fractions showed scarcely activities.After 48 h,the inhibition rates of the first ?the second ? the third and the forth fraction were 100%,completely.After 72 h,the inhibition rates of the third and the forth fraction were 100%,completely,and the first and the second fraction were 81.95% and 76.39%,respectively.4.The ingredient of K2 ? K3 ? K4 is different from the main four flavone,orientin,isoorientin,vitexin and isovitexin,by TLC.The main components of K5?K6?K7 are isoorientin and isovitexin.The active component S10 was collected from K10 by gel column chromatography.On the basis of UV,NMR,MS data,the structure of S10 was indentified.Every sign points to the fact that this compound is Trans-p-hydroxy cinnamic acid.The antifungal activity of S10 against Gibberella sanbinetti was examined,while compared with the standard P-hydroxy cinnamic acid.The results showed that,after 72 h,the inhibition rates of S10 and the standard were 83.43% and 81.76%,respectively.The EC50 of trans-p-hydroxy cinnamic acid and S10 to Gibberella sanbinetti were 10.80mg/L and 11.02mg/L.So author consider that Trans-p-hydroxy cinnamic acid is the important antibacterial component.