Investigation Of The Avian Mycoplasma Infection In Dead Chicken Embryos During Late Stage Of Incubation In Some Broiler Breeder Farm

The egg infection often is main reason of avian mycoplasmosis passed from one generation to the next, so the control of avian mycoplasma infection should first do a good job in building disease in chickens. But the variation characteristics of the Mycoplasma itself, immune escape function determines the avian mycoplasma disease is more difficult to control than the acute, virulent infectious disease, through the vaccine immunity and drug treatment is very difficult to eliminate the mycoplasma. Among all kinds of measures of prevention and control of mycoplasma, immune especially live vaccine immunization is still the core and focus of comprehensive control. In order to understand the current domestic species of avian mycoplasma control effect. This study of a breeder flocks in late hatched embryos died of avian mycoplasma infection investigation and screening of sensitive drug, in order to put forward more perfect chickens Mycoplasma prevention and control measures.Isolation of mycoplasma is difficult, which mainly lies in the separation of sample selection. For the dead embryo in avian mycoplasma isolates to select samples with no domestic report. To determine the dead embryo avian mycoplasma best separated parts. The artificial infection embryo, after the death of the chick embryo were selected yolk sac, chorioallantoic membrane, egg yolk, oropharyngeal secretions, lung of MG were isolated and cultured, and ultimately determine the optimal separation parts of the embryo death as yolk sac.In order to understand later hatching stage dead embryo in avian mycoplasma infection, this study from Shandong, a suspected avian mycoplasma infection of chicken samples were collected, by simultaneous detection of dead embryos in avian mycoplasma isolation culture and PCR to determine the avian mycoplasma infection rate, evaluation of avian mycoplasma prevention and control effect, and avian mycoplasma clinical isolated strains and drug resistance analysis. Isolation and culture of 3 strains of MG, not isolated to MS. According to the MG and 16 S r RNA gene gene MG MGc2 conservative region synthesized two pairs of specific primers and PCR detection of 100 samples, respectively, according to the electrophoresis and PCR products were sequenced and the results, final judgement the breeding field of MG infection rate was 59%.MIC of 14 kinds of antimicrobial agents against 12 strains of clinical isolates with obvious blood coagulation were determined.. Antibiotic susceptibility test results showed that valnemulin hydrochloride ? tiamulin fumarate and doxycycline have the highest sensitivity and the average value of MIC were 0.028 ?g/m L, 0.167 ?g/m L and 0.29 ?g/m L, respectively. followed by spectinomycin hydrochloride, water-soluble florfenicol, tylosin tartrate, levofloxacin hydrochloride and enrofloxacin hydrochloride, the average value of MIC is between 2.5 ?g/m L and 10 ?g/m L, the lower were lincomycin hydrochloride, water-solubility azithromycin, norfloxacin lactate,amikacin sulfate, tilmicosin phosphate and kitasamycin tartrate, the average value of MIC is between 20 ?g/m L and 160 ?g/m L.