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Genetic Analysis Of Flag Leaf Related Traits Using A RIL Population Derived From Yunnan × Yanzhan 1

Posted on:2017-06-06Degree:MasterType:Thesis
Country:ChinaCandidate:T Y DingFull Text:PDF
GTID:2323330485957505Subject:Crops
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Wheat(Triticunm aestivum L.) is the most important food crops in our country and even the world. In our country there are more than 35 percent of wheat-based food and manufactured goods. Common wheat heterologous hexaploid(2n = 6x = 42), including A, B and D 3 genomes, the genome is huge. Most wheat and other important crops of agronomic traits(traits such as yield, quality traits, resistance, growth period, etc.) is usually by multiple genes of quantitative trait loci(quantitative trait locus, QTL) control using conventional methods for these choose low efficiency traits. Breeding of high yield and good quality varieties has been one of the main tasks of wheat breeding, along with research and development in molecular genetics and genomics, molecular markers and targeting methods for wheat Main Agronomic Traits of law provides an effective means, for fine mapping and cloning genes related to lay the foundation for the study of genetic markers greatly promoted the development of the QTL. In this study, Yunnan wheat and Yanzhan No. 1 Construction of recombinant inbred lines, multi-planting years, the use of 90 K chip technology, build high-density genetic linkage map of wheat, grain weight of the flag leaf and genetic analysis, the control of the flag leaf and grain weight related traits QTL fine mapping, to understand their position and the effect on the chromosome, molecular marker-assisted selection in wheat breeding, molecular breeding and polymerization Pictured important cloning important.The main results were as follows:(1) In 3 different environments in a year, flag leaf area, which flag leaf length, flag leaf width and grain weight data show that: the coefficients of skewness and kurtosis less than 1 of the above traits about Yanzhan No. 1 wheat and Yunnan,so they can be used for localization QTL. Wheat flag leaf length, flag leaf width and flag leaf area,those traits very significant level, flag leaf length and grain weight correlation reached significant levels only in dezhou's environment, there is no significant relationship between other traits. The flag leaf length, width and relevance of wheat flag leaf area and grain weight did not reach significant levels in the groups.(2) We locate 1416 molecular markers on 21 chromosomes eventually. The map included 24 linkage groups, covering 21 wheat chromosomes, covering a total length of chromosome 3663.24 c M, the average distance are 2.59 c M between markers.In the Genetic linkage map, A genome contain 549 markers(38.77%), A genome is 1364.39 c M, genetic distance is 2.49 c M between two markers; B genome contain 580 marks(40.97%), B genome is 1158.88 c M, The genetic distance is 2.00 c M between markers; D genome contain287 markers(20.27%), D genome is 1140.00 c M, genetic distance is 3.97 c M between markers. As for single chromosome, the 3B chromosome have greatest number of markers-118 markers; 4D chromosome has least marks, only contions 11 markers; chromosome 7A covering longest length of the chromosome, which are 359.03 c M, 4B chromosome cover the shortest for 108.01 c M.(3)We locate QTL in 3 different environments in a year, for flag leaf area, flag leaf length, flag leaf width and thousand grain weight, we detected 17 sites related flag leaf under 3 environments, it located in 1A?1B?1D? 2A? 2B? 3A? 3B? 4B?4D? 5A? 5B? 5D? 6A? 6D? 7A and 7D chromosome, a single QTL phenotypic mutation rate in 6.82%-32.87%, different chromosomes have different numbers of QTL, it concentrates in 1 and 5 homologous group. we detected 56 QTL related flag leaf area, single QTL explain phenotypic variation between 5.78%-53.27%. we detected 5 QTL related thousand grain weight, QTGW-sdau-5A can be detected in two environments E1 and E2, QTGW-sdau-1A can be detected in two environments E1 and E3, the remaining sites only are detected in a single environment. It can be the same site in different environments, QFLL-sdau-5A and QFLW-sdau-5A is the same site in E1 environment; QFLL-sdau-1A and QTGW-sdau-1A is the same site in the E2 environment; QTGW-sdau-1B in E3 environment and QFLW-sdau-1B in the E1 environment are the same site; QFLW-sdau-5A in the E2 environment and QTGW-SDAU-5A in the E2 environment are the same site.
Keywords/Search Tags:Wheat, Recombinant inbred line population(RIL), Quantitative trait locus(QTL), Genetic map
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