| Mastitis is one of the important diseases that affect the development of dairy industry,and cause huge economic losses to cattle farming. Staphylococcus aureus is the main pathogens of mastitis, and now the emergence of antibiotics resistant strains has brought great difficulties to the treatment of the disease.Adhesion play an important role in the process early infection of Staphylococcus aureus, FnBPA is one of the most important adhesion. In this study FnBPA-A different genetic polymorphism recombinant plasmids of Staphylococcus aureus used as research object,our aim is to explore the relationship of FnBPA-A genetic polymorphism and its immune biological characteristics.In this study we analyzed the adhesion factor FnBPA-A sequence of Staphylococcus aureus, and used constructed FnBPA-A different genetic polymorphism recombinant plasmids to immunized mice, analyzed its biological characteristics.The results showed that the antiserum immunized with different FnBPA-A genetic polymorphism recombinant plasmids have different ability to recognize different Staphylococcus aureus isolates,different genetic recombinant plasmids of FnBPA-A has Fg adhere inhibition but it was not significant(P<0.05).Different FnBPA-A genetic polymorphism recombinant plasmids can enhance Lymphocyte proliferation and IFN-? level. The immune protection was different between different FnBPA-A genetic polymorphism recombinant plasmids,and it was corelation with celluar immune level.Tissues explant culture were used to culture bovine mammary epithelial cells, and purified mammary epithelial cells could be obtained by trypsin digesting. The expression of cytokeratin 18 genes was positive in cultured cells, it proved that these cultured cells were the mammary epithelial cells.And then detected the growth curve of purified cells.CFSE and DiI were used staining on Staphylococcus aureus and cultured mammary gland epithelial cells, then the adhesion that Staphylococcus aureus to the cow mammary gland epithelial cell observed under laser confocal microscope. The ability of 4 different Staphylococcus aureus isolates adhesion to the bovine mammary gland epithelial cell were compared, the results showed that 4 strains have different adhesion ability. Among them the adhesion ability of GY278 is strongest and is significant to other isolate strains(P<0.05).Then use cultured bovine mammary epithelial cells we analyzed the impact of antiserum from rabbits immunized with FnBPA-A different genetic polymorphisms recombinant plasmids inhibited 4different Staphylococcus aureus isolateds adhesing to cattle mammary gland epithelial cells. It was found that different antiserums have different ability to inhibit adhesion.In conclusion, FnBPA-A different genetic recombinant plasmids of Staphylococcus aureus has the different immune characteristics, This research provides foundation for Staphylococcus aureus prevention and immunology. |
PDF Full Text Request