The Study Of The Japanese Camellia Tissue Culture Of Plantlet Regeneration

In order to solve the Japanese camellia introduce difficulties,seeds and seedlings source shortage,difficult to meet the demand of forestry construction and building of ecological civilization,With camellia leaves and stem segments as explants,study its regeneration plant system,and acquire the improvement of camellia best explants disinfection method and time,and effectively relieve camellia material Browning.To establish the Camellia regeneration system culture conditions,and Camellia callus induction,proliferation and differentiation,the best ratio of hormone,stem section bud induction and bud proliferation,the best ratio of hormone,regeneration plant roots.The main results were as follows:(1)Camellia explants disinfection combination and the best timeSelection of camellia plant growth,shearing new leaves and branches tender tip of the stem section 30 min and clean with water wash away the dust,and then further cleaned with detergent,detergent residues in the tap water is rinsed clean again,and then placed in a sterile room ultra clean bench,clean with sterile water for 1 min,then use 75% ethanol for 20-30 s,with sterile water flushing after 2 times,add 0.1% Hg Cl2,not shake when disinfection cylinder,timing 4 to 8 min,take out after rinse 4-5 times with sterile water,placed in a sterile filter paper blot moisture,inoculation in different plant growth regulator on the MS culture medium of additional do original culture.(5)Camellia callus induction,proliferation and differentiation of the best ratio of hormonesMS+6-BA 2.0 mg?L-1+2,4-D 0.5 mg?L-1medium is the best medium for new blade callus induction,MS+6-BA 15mg?L-1+IBA 0.5 mg?L-1is the best blade inducing callus proliferation medium;MS+6-BA 2mg?L-1+NAA 0.2 mg?L-1+ZT 2.0mg?L-1is best medium induction of callus of tender stem section,MS+6-BA3mg?L-1+NAA 0.2 mg?L-1+IBA 0.5 mg?L-1is the best medium for callus differentiation;The callus induced by stem section has the adventitious buddifferentiation potential.(6)Stem section bud induction and bud proliferation of optimal ratio of hormonesIn add 6-BA 2.0 mg?L-1+ IBA 0.5 mg?L-1MS induction medium,explant for tender stem section of camellia,bud induction rate reached 97%,MS+6-BA2mg?L-1+NAA 0.15 mg?L-1+ZT 2.0 mg?L-1,MS+6-BA 3mg?L-1+IBA 0.5mg?L-1+NAA 0.5 mg?L-1for adventitious bud multiplication culture.(7)The root of camellia plantsJapanese camellia adventitious bud rooting induction method is outside the tube filter paper bridge root method,the technology in the temperature of 20-30 ?,humidity is 85% ~ 85%,the talent base under the hormone NAA or IBA 1000 mg/L for 20 min,use 1/5 ms culture medium,root culture box production,can improve the rooting rate of seedlings,seedling growth.