| In order to study the 15%,30% feed restriction on sanhuang chickens growth performance,immunological characteristic and gene expression in the spleen,in this study,mainly carried out from the following two parts.Test one Effects of Feed Restriction on Growth Performance and Immunological Characteristic of Sanhuang ChickensThe 72 female Sanhuang broilers at 15 day-old were fed in this experiment.The broilers were randomly assigned to 3 treatment groups: ad libitum(control group),15% feed restriction(trial group 1)and 30% feed restriction(trial group 2),24 in each group.Each bird raised in one cage,chickens were slaughtered after feed restriction for 15 days(30-day-old),compensatory growth for 14 days(44-day-old),compensatory growth for 35 days(65-day-old),respectively.Different levels of feed restriction on sanhuang chickens growth performance,slaughter traits,visceral index,immune organ index,peripheral blood lymphocytes were studied.Following was the results:(1)After 15 d of feed restriction in sanhuang chickens,final weight and ADG of the trial group 1 and 2 were significantly lower than the control group(P<0.05),and ADG of the trial group 2 had a significantly lower than trial group 1(P<0.05);The F/G of the control group and the trial groups showing no significant difference(P>0.05);After 35 d of compensation growth,ADG and TFI between three groups showing no significant difference(P>0.05),the F/G of trial group 2 was significantly lower than trial group 1(P<0.05);The whole phase of feed restriction-compensation growth,the final weight,ADG and F/G between three groups showing no significant difference(P>0.05),but the F/G of the trial group 2 was lowest.(2)15 d of feed restriction in sanhuang chickens increased the rate of breast muscle of trial groups,the rate of breast muscle of trial group 1 was increased by 3.4%,no significant difference(P>0.05),the rate of leg muscle of trial groups were decreased,no significant difference(P>0.05);After 15 d of feed restriction,eviscerated weight and semi-eviscerated weight of the trial groups were significantly lower than the control group(P<0.05),the eviscerated weight and semi-eviscerated weight of trial group 2 was significantly lower than trial group 1(P<0.05),eviscerated ratio and semi-eviscerated ratio between three groups showing no significant difference(P>0.05).(3)After 15 d of feed restriction in sanhuang chickens,heart weight and liver weight of the trial group 1 and 2 were significantly lower than the control group(P<0.05),heart index and liver index between three groups showing no significant difference(P>0.05),pancreas index of the trial group 1 and 2 were significantly higher than the control group(P<0.05);After compensation growth,heart weight,liver weight and pancreas weight between three groups showing no significant difference(P>0.05).(4)After 15 d of feed restriction in sanhuang chickens,spleen index of the trial group 1 was significantly higher than the control group(P<0.05),thymus index and bursal index between three groups showing no significant difference(P>0.05).(5)After 15 d of feed restriction in sanhuang chickens,blood CD3+ lymphocyte of the trial group 1 was significantly lower than the control group(P<0.05),blood CD3+ lymphocyte of the trial group 2 was significantly higher than the control group(P<0.05),blood CD8+ lymphocyte of the trial group 1 and 2 was significantly lower than the control group(P<0.05),blood CD4+ lymphocyte between trials groups and control group showing no significant difference(P>0.05).This study shows that a moderate amount of feed restriction,can be improved the growth performance of sanhuang chickens,promoted the spleen index,improved immune function,and achieve a good degree of compensation effect.Test two Effects of Feed Restriction on Spleen Gene Expression of Sanhuang ChickensIn order to study the feed restriction on sanhuang chickens gene expression in the spleen,this experiment chose of 30-day-old,44-day-old,65-day-old healthy female sanhuang chicken spleen tissue,each period and each group 6 samples in feed restriction and freedom group,a single individual for the repeat units.According to the candidate genes(COL1A1,MRPL45,DAD1,ZNF644)and related sequences,using fluorescence quantitative RT-PCR carries on the quantitative analysis of candidate genes and related series.Following was the results:(1)At the age of 30-day-old,gene expression of COL1A1,DAD1,MRPL45,ZNF644 of the trial group 1 and 2 were higher than the control group,the trial group 1 is 1.26 times,1.04 times,1.35 times,3.61 times of the control group,respectively;the trial group 2 is 2.22 times,1.47 times,1.26 times,2.81 times of the control group,respectively.(2)At the age of 44-day-old,gene expression of COL1A1,DAD1,MRPL45,ZNF644 of the control group and trial group 1 were higher than the the trial group 2,the control group is 2.10 times,1.12 times,1.09 times,1.92 times of the trial group 2,respectively;the trial group 1 is 3.16 times,1.11 times,1.89 times,2.51 times of the trial group 2,respectively.(3)At the age of 65-day-old,gene expression of COL1A1,DAD1,MRPL45,ZNF644 of the trial group 1 and 2 were higher than the control group,the trial group 1 is 2.30 times,1.46 times,1.42 times,1.73 times of the control group,respectively;the trial group 2 is 3.81 times,2.14 times,2.85 times,5.50 times of the control group,respectively.In this study,we identified differences in feed restricted sanhuang chickens spleen related immune genes through quantitative analysis.This provided important information for further study of feed restriction on sanhuang chicken immune function,and provided a reference for human researching feed restriction effects in broiler from the molecular level. |
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