Germplasm Resources Genetic Diversity And Main Agronomic Trait Analysis Of Flammulina Velutipes

Flammulina velutipes(Fr.) Sing is an edible and medicinal fungi, which most cultivated in factory farms, and with its rich nutriention and delicious taste attract a lot of people. However, studies have reported on the germplasm diversity are very little. In this paper, we take Flammulina velutipes(Fr.) Sing as the research object, and then using SSR markers to analysis DNA diversity among the tested strains, and some of its relations with important agronomic traits. After statistical data analysis and cluster analysis of the results, we find that there are abundant genetic variation of Flammulina velutipes(Fr.) Sing germplasm resource.This analytical method combine of modern and traditional methods for the analysis of genetic diversity overall, the system provides a theoretical and practical basis, provide a valuable reference for other species polymorphism studies also strengthen germplasm collection and gene mining research foundation, and new breeding work to provide guidance to improve the efficiency of germplasm into.A total of designed and synthesized mushroom 207 pairs of SSR primers, and obtained by screening a total of 68 pairs of high polymorphism, deletion low mushroom SSR primers. Use the R language, We did primers polymorphic information analysis, 9 pairs primers derived allele frequencies are 0.076923077,But there are differences in their alleles,Range between 7 to 13, the average number of alleles per primer pair is 10; Allele most primers FS11, FS27, FS28;lele least seven primers, which are FS93, FS82, FS144.Polymorphism information content between 0.60687-0.90663, an average of 0.801911866,And from the table we can see substantially more number of alleles, PIC greater the value.And based on the analysis of the results did UPGMA cluster analysis, 8.4 stars genetic distance,The three wild strain G4, G6, G11 and the remaining 33 strains into two categories.he genetic distance 7.4 turn 33 strains into two groups white yellow. SSR markers population structure analysis tested strains into seven groups.This study use stucture software tested 36 mushroom germplasm genetic structure, drawn ethnic 1 contains three resources: G14, G11, G17, the group 2 contains five resources: G51, G47, G49, G46, G52, ethnic 3 contains 10 resources: G40, G41, G50, G45, G29, G56, G39, G35, G44, G42, the group 4 includes four resource: G4, G6, G8, G5, ethnic 5 contains five resources: G18, G28, G32, G15, G12, the group 6 contains six resources: G13, G21, G24, G25, G20, G22, the group 7 contains three resources: G7, G16, G27.This study combined with agronomic traits phenotype correlation analysis, and based on the population structure analysis and cluster analysis, can be concluded that the primers Fs109 and Fs115 can be the specific primers of distinguishing cop colors traits; primer Fs83 can be the specific primers of distinguishing stipe diameter trails.