Assoiciation Of Polymorphisms In PRLR?GDF9 And BMPR-IB Gene With Reproduction Performance In Zongdihua Pig

In the study, Zongdihua pig was regarded as experimental subjects. The technology of DNA pool and DNA direct sequencing were used to analyze the genetic polymorphisms of PRLR, GDF9 and BMPR-IB gene. Analysis of the correlation between the polymorphisms of PRLR, GDF9 and BMPR-IB gene and total number born, number born alive, average birth weight of piglets and lactescence. The aims of this study were to provide significant experimental foundation of molecular marker-assisted selection for the improvement of reproductive traits of Zongdihua pigs. The results were as follows:1. The 6 SNPs sites of PRLR gene were detected in the Zongdihua pigs groups, C1115 G, A1528 T, G1600 A, C1620 T and A1789 T were missense mutations. The C1115 G site led to the consequent change which caused proline into arginine. Association analysis indicated that the effect of polymorphism in the C1115 G site of PRLR gene on the traits of total number born, number born alive and lactescence were high significant(P<0.01). The A1528 T site led to the consequent change which caused arginine into tryptophan. Polymorphism of A1528 T site on the traits of total number born was high significant(P<0.01) and number born alive was significant(P<0.05). The G1600 A site led to the consequent change which caused glycine into cysteine. Polymorphism of G1600 A site had no significant effect on the reproductive traits of Zongdihua pigs(P >0.05). The C1620 T site led to the consequent change which caused alanine into serine. Polymorphism of C1620 T site on the traits of total number born, number born alive and lactescence were significant(P<0.05), and the site on the traits of wasn't significant(P?0.05). The A1789 T site led to the consequent change which caused serine into cysteine. Polymorphism of A1789 T site had significant effect on total number born, number born alive and average birth weight of piglets were significant(P<0.05). But the A1283 C site was synonymous mutations. Polymorphism of A1283 C site on the traits of total number born, number born alive and average birth weight of piglets were high significant(P<0.05).2. The 2 SNPs sites of GDF9 gene were detected in the Zongdihua pigs groups. The C1009 T and T1014 C sites led to the consequent change which caused aspartate into histidine and arginine into tryptophan. Polymorphism of C1009 T site of GDF9 gene had high significant effect on total number born(P<0.01), and the site had significant effect on total number born, number born alive and average birth weight of piglets(P<0.05). Polymorphism of T1014 C site of GDF9 gene had significant effect on total number born, number born alive and average birth weight of piglets(P<0.05).3. The 2 SNPs sites of BMPR-IB gene were detected in the Zongdihua pigs groups. The G595 C site was missense mutations. The G595 C site led to the consequent change which caused aspartate into histidine. But the C643 T site was synonymous mutations. Polymorphism of G595 C site of BMPR-IB gene had significant effect on total number born and number born alive(P<0.05), and the site had significant effect on. Polymorphism of C643 T site of BMPR-IB gene had high significant effect lactescence(P<0.01).