The AMF And Chitinolytic PGPR Selecting And Its' Dual Inoculation Effect On Maize Growth

In recent years, the "decrease application rate & increase efficiency of fertilizer" has become the important subject to the maize producer in jilin Province. Many study has demonstrated that AM Fungi and PGPR could promote nutrient uptake through Symbiotic role. Either single use of AM fungi or PGPR obviously increased host plants' growth and yield, while their are few reports on AM fungi and chitinolytic PGPR application in maize at field. Therefore, soil samples were collected from the different corn cultivation area and AM fungi and PGPR strains have dominant populations were isolated for following study. Nine strains of AM fungi and 5 strain of PGPR were identified by microscope observation and their gene sequence analysis, respectively. Finally, 8 strains of Glomus and one strain of Acaullospora was discovered and the PGPR was identified as Bacillus sp. according to its 16 s r RNA gene sequence. After fermented with mixed materials, the population of living bacterium is as high as 8.82 Log CFU/g. As well as, the number of AM spores and infection rate was reached to 1772/20 ml and 82.5% respectively after propagated with clover as host plants. in vitro investigation showed that Bacillus sp. can produce chitinase, indole acetic acid. Furthermore, pot trails well proved its metabolites can promote crops on the absorption of nutrients. Our study proved excellent combination and performance ability of G. intraradices within PGPR compost and select as dual-inoculation inoculum with Bacillus sp. for field trials. Field experiments indicated that the yield decreasing range was controlled within 4% in the dual-inoculation amendment under the premise of chemical fertilizer decreased by 25% compared with the conventional fertilization amendment. All these results provides reference for the large-scale application in the field of this research in the future.