| Rice(Oryza sativa L.) is one of the most important staple crops in the world. Promoting rice yield and quality is of particularly important for the food security and the human diet demand. Here, the rice single segment substitution lines(SSSLs), which generated by using the rice variety Huajingxian 74 as receptor, were used for the molecular design breeding. Certain novel rice varieties with high yield and quality were identified and characterized through the examination of the length of subsitituted fragments and phenotypic investigation of agronomic traits, as well as analysis of rice quality triats. Our results are summarized as following:Fourty six SSSLs containing either single or multiple segments were used for the comparative analysis in both early and later seasons of 2015. In term of these 46 SSSLs, there are 3 single SSSLs containing single segments, while other 4, 6, 12, 14 and 7 polymerized SSSLs contain triple, quadruple, quintuple, sextuple and septuple segments, respectively. Twenty eight substituted segments containing distinct genes were identified by charactering the phenotype and genotype of above SSSLs that evaluated in both early and later seasons. These substituted segments with 19.79 c M length in avarerage were derived from 23 various donors and referred to 21 associated genes. Our results showed that 41 novel SSSLs contain Wx gene controlling amylose content, 35 novel SSSLs contain GS3 controlling grain length, 34 novel SSSLs contain Hd-1-2 controlling high yield and early mature, 19 novel SSSLs contain Gwt-10-3 controlling high yield, 19 novel SSSLs contain ALK, 17 novel SSSLs contain CRE controlling rice cooking elongation, 14 novel SSSLs contain fgr controlling fragrance, 13 novel SSSLs contain Gl-2-1 controlling grain thickness and length, 12 novle SSSLs contain q BLAST-11-2 controlling blast resistance and 9 SSSLs contain gw8 controlling grain width, respectively.In the early season of 2015, 34 novel SSSLs and two controls, including Huajingxian 74 and Shengliangyou58 xiangyouzhan varieties, were used for the comparative analysisThe line L151-02(23-06//19-07/08-03P04), L151-21(23-06/23-11//03-08/03-03///09-01// 11-08/11-12) and L151-28(23-06/15-08/08-03/07-10//22-02/19-07/08-03/11-01/07-10) withsuperior agronomic traits and quality traits were obtained. Among above 3 SSSLs, In the late season of 2015, 22 novel SSSLs from early season, 12 novel SSSLs supplemented in late season and two controls, including Huajingxian74 and Yuejingsimiao2 hao varieties, were used for comparative analysis. The line L152-4(15-06/15-03//17-08///11-01 P02-1), L152-29(15-06/11-01/09-01/11-08/11-12//11-02/11-03), L152-30(15-06/15-03/11-01/ 09-01/11-08/11-12//11-02) and L152-32(20-06/15-08/19-07/11-01/07-10/03-08/03-03//23-10) with superior agronomic traits and quality traits were obtained, whereas the L152-29 achieved the level 2 of national standard of rice(GB/T 17891-1999).In summary, we propose that the L151-02(L152-02), L151-21(L152-28) and L152-01 are the potential material for further rice design breeding, The L151-02(L152-02), L151-21(L152-28) and L152-01 exhibited superior phenotype in multiple traits among the 22 SSSLs that evaluated in both early and later seasons. our results revealed that the heading date of them was 87.19, 86.49 and 88.69 days, respectively, the average ratio of length to width of them was 3.33, 3.36 and 3.18, respectively, the percentage of entire white rice of them was 45.64%, 48.13% and 44.21%, respectively, the percentage of amylose content of them was 22.50%, 23.47% and 23.02%, respectively, and the average effective production of them was 347.83 kg/666.7m2, 326.92 kg/666.7m2 and 321.73 kg/666.7m2. those novel SSSLs could be evaluated in the provincial regional test of rice. |
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