Molecular Characterization And Expression Analysis Of TaTAC1 Gene In Triticum Aestivum

Tillering angle affects plant population structure, photosynthetic efficiency and morphogenesis, and ultimately affects the yield and quality. But studies on tillering angle have been rare reported in wheat.Studies of tillering angle had rare reports in wheat. To resolve the expression patterns of TaTAC1, having a preliminary understanding of the molecular genetic mechanisms of this gene and its genetic relationship with the tillering angle, this study used CN16,SM969,Lan2399,SHW-1 as materials and homology-based cloning to separate TaTAC1. We used bioinformatics software to analyze the characteristic of TaTAC1 sequence and applied real-time fluorescent quantitative PCR to analyze its expression pattern.In order to research the function of TaTAC1, this study carried out the subcellular localization experiments.The results are as followed:1. In wheat varieties have already included two TaTAC1 cDNA sequences.The length of first category cDNA was 1118 bp, including 780 bp ORF and 338 bp 3 'UTR. ORF encoded 260 amino acids. The length of second cDNA was 1143 bp. including 780 bp ORF and 363 bp's 3'UTR.ORF encoded 260 amino acids. there was a base mutation at No.10 of the cDNA sequence in CN16-2 and SM969-2.which causes to terminate in advance so as to gene expression being block. In addition, there were 6 bases insert "CGCGCG" in 109-115 base location that led to protein ?-pleated sheet decreasing.2. Amino acid sequence of TaTAC1 was similar to OsTAC1, ZmTAC1 and SbTACl.The Similarities were 75.95%,62.02%.65.04% respectively. There were highly conserved amino acid sequences with five areas, within the area I exist "IGT" structure, which ?, ?, ?, ? area were at the same conservative with IGT gene families.3. molecular weight of TaTAC1 was 29033.2, isoelectric point was 5.42.Among them there exsited the polynucleotide and protein secondary structure of specific binding sites, alpha helix, extending chain and random curl and a clear across the membrane structure field.4. Real-time fluorescent quantitative PCR analysis showed that TaTACl in leaf sheath, stem had efficient expression of tillering stage, tillering node, leaf and root expression quantity minimum.SPSS20.0 analyzed the gene expression in tillering node of each period and tillering angle had significant positive correlation. Pearson correlation coefficient was 0.677;Other organizations expression quantity and tillering angle had no significant correlation.5. TaTAC1 had subcellular localization in the cell membrane. TaTACl was expressed under different organization in different periods.It expressesd specificity organize time and space. The gene expressed in leaf sheath, stem, tillering node efficiently. Location expression quantity of tillering node was associated with a significant phenotypic and protein in the cell membrane. Thus speculate TaTAC1 had positive regulation with tillering angle at the mRNA level, and it may be involved in auxin polar transport process to change the size of tillering angle.