Cloning And Expression Analysis Of Tatfl1 Gene In Triticum Aeativum L.

Wheat is an important food crop,and its yield level occupies an important position in the national economy.But wheat's large and complex genome because it is a heterogenous hexaploid has slowed the research progress of its functional genes.The development of wheat inflorescence is closely related to the yield,and the number of spikelet per ear which is determined by inflorescence development is an important determining factor of wheat yield.To analyze the biological function of the genes related to the development of wheat inflorescence,and to clarify its practical function has definite theoretical significance and practical application value for wheat yield research.Terminal Flower 1(TFL1)and its homologous genes are the feature genes of the inflorescence meristem,which may play an important role in controlling the characteristics of inflorescence meristem and delaying flowering.This study used Chinese spring as experimental material,three wheat homologous gene of TFL1 and its promoter sequences has been cloned.Meanwhile,the bioinformatics was used to analyze the molecular properties and the relationship three genes with the homologous genes between different species.The expression specificity of TaTFL1 was analyze through the qRT-PCR.In addition,the gene of TaTFL1-5A has also been transferred to arabidopsis thaliana to preliminarily understand its function.1.We cloned the cDNA fragments of TaTFL1-5A,5B,5D in common wheat.The results from bioinformatics analysis show that three sequences are contains an open reading frame encoding 173 amino acid residues of protein that have typical TFL1 protein structure.TaTFL1-5A,TaTFL1-5B and TaTFL1-5D proteins are hydrophobic proteins and no signal peptides,which are located in cytoplasm.The homology comparison and evolutionary tree analysis of TaTFL1 protein showed that TaTFL1 was closely related to monocotyledons.The promoter analysis shows that there are a large number of light response elements in the promoter,and a CAT-box which is related to the meristem expression.2.The results of qPCR analysis showed that TaTFL1 was expressed in all tissues.The expression quantity in the leaves was lower and in the stem of pre-bloom was the highest.in addition,the expression quantity of both were significantly higher in pre-bloom than post-flowering.There is also a weak expression in the buds,glumes and grains.The result of expression quantity in different development stages of the young inflorescence indicated that expression quantity in 1 to 2 cm of young inflorescence was highest and showed a downward trend as the growth of young inflorescence.Young inflorescence has always maintained a high expression level when compared with other organization.3.The results of arabidopsis genetic transformation displayed that TaTFL1 has certain control function from vegetative growth to reproductive growth.The gene can make arabidopsis thaliana flowering delay,its flowering time delayed 15 days compared with wild-type Arabidopsis.But TaTFL1 didn't like that overexpress TFL1 gene in arabidopsis leads to change the morphological structure of the flower.The number of branches in main stem increased,which can be speculated that the TaTFL1 gene may be closely related to the number of spikelets in wheat.