Relationship Between Calves' Birth Weight And Some Adipokines In Cow's Venous Blood, Cord Blood And Placenta

Birth weight is vital for evaluation of calves intrautherinegrowth and productive performance after adulthood. Previous studies have demonstrated the significant correlation between birth weight and concentration of adipokines inpregnancy venous blood, placenta and cord blood, indicating adipokines are involved in regulation of fetus development. Research in correlation between calves' birth weight and adipokines in cow's venous blood, placenta and cord blood has not been reported so far.In order to explore the correlation between calves'birth weight and adipokines in cow's venous blood, placenta and cord blood; 54 healthy Chinese Holstein cows that are 500kg of weight, experienced 2-4 full-term natural birth were used, in which, cows given birth to calves that are<40kg were included in group A (n=9); that are between 40kg-45kg were included in group B (n=25);that are?45kg were included in group C (n=20). Calves' birth weight and gender were recorded.Venous blood was collected before labor, placenta and cord blood of cows were collected after birth. ELISA was used for evaluation of adiponectin, leptin, visfatin, insulin and IGF-1 in venous blood and cord blood. ELISA and RT-PCR were used for evaluation of adiponectin, leptinand visfatin in placenta.The correlation among different adipokines in venous blood, placenta and cord blood of cows, as well as the correlation between adipokines and calves'birth weight were analyzed, potentiating further research in mechanisms of adipoknie's regulation of calves'birth weight.1.The relationship between adipokines in venous blood and calves'birth weight. We showed (1) There is a positive correlation among adiponectin, leptin, visfatin, insulin and IGF-1 in venous blood (p<0.05); there are significant positive correlation between leptin and adiponectin, leptin and visfatin, adiponectin and insulin, visfatin and insulin, IGF-1 and adiponectin, IGF-1 and leptin, IGF-1 and visfatin, IGF-1 and insulin (p<0.01). (2) There is no significant correlation between calves'birth weight and adiponectin, leptin, visfatin, insulin and IGF-1 in venous blood (p>0.05), but adiponectin and insulin are trending to decrease as calves'birth weight increases.2 The relationship between adipokines in placenta and calves' birth weight. We showed (1) There is a significant positive correlation among the mRNA and proteinofadiponectin, leptin, visfatin in placenta (p<0.01). (2) The mRNAof adiponectin in placenta is positively correlated with calves'birth weight (P<0.05). The mRNAof leptin in placenta is significant positively correlated with calves' birth weight (p<0.01).The protein of adiponectin and leptin in placenta are significant positively correlated with calves'birth weight (p<0.01).There is no significant correlation between calves' birth weight and the mRNA and protein of visfatin in placenta (p>0.05).3 The relationship between adipokines in cord blood and calves' birth weight. We showed (1) There is a significant positive correlation among adiponectin, leptin, visfatin, insulin and IGF-1 in cord blood (p<0.01), in which, insulin are positively correlated with adiponectin and visfatin (p<0.01), insulin are positively correlated with leptin (p<0.05), visfatin and leptin are positively correlated (p<0.05), visfatin and insulin are positively correlated (p<0.05), leptin are positively correlated with adiponectinvisfatin and leptin are positively correlated (p<0.01). (2) leptin in cord blood are positively correlated with calves' birth weight (p<0.05), while there are significant positive correlation between calve's birth weight and adiponectin.visfatin, insulin, IGF-1(p<0.01).Conclusion:(1)There is no significant correlation between adiponectin, leptin, visfatinin cow's venous blood and calves' birth weight(p>0.05). (2)Adiponectin and leptin in placenta are positively correlated with calves' birth weight(p<0.01). (3)There are significant positive correlation between calve's birth weight andadiponectin, visfatin in cord blood (p<0.01), while leptin in cord blood are positively correlated with calves'birth weight(p<0.05). (4) There are no significant correlation among adiponectin, leptin, visfatin in cow's venous blood, placenta and cord blood (p>0.05).