The Preliminary Localization Of Oat Dwarfing Gene DwWA

The genus Avena L. belonging to Aveneae, Pooideae, Gramineae, including about 29 species. Oat is a very important cereal both for human and animals, and is also one of the eight major food crops, which is widely cultivated in the world. It has high nutritional and economic value, the dietary fiber beta glucan in its grain plays an important role in reducing cholesterol, meanwhile, it also contains essential mineral elements and a variety of trace elements. Oat is barren, cold tolerance and drought resistance with low agricultural risk coefficient, and has no competitiveness with other cereals such as rice and wheat for land. Therefore, oat occupies an important position in Chinese grain production. But there are many problems in the production practice of oats at present. The oat plant is too high, easy to lodging, resulting in low yield and poor quality. So, to explore the oat dwarfing gene and cultivating new varieties of lodging resistance is the fundamental way to solve the problem of lodging and improve the yield. Two oat varieties that are very different in plant height were used to create a mapping population for exploring new dwarfing gene in oat and screening dwarf related markers. The main results are summaried as follows:1.A mapping population of 306 F2 individuals used in this experiment was derived from a cross between two early, high-quality, hexaploid cultivated oat ?A. sativa L.? genotypes 'WAOAT2132' and 'Caracas' which provided by Agriculture and Agri-Food Canada, and a mapping population varying in height was generated.2.102 SSR markers which were reported in previous studies were derived from wheat and barley genome for preliminary locating of the dwarfing gene contained by WAOAT2132. Of these SSR markers, four named AME013?AME117?AME055 and OL0256 respectively were polymorphic in parents and tall and dwarf DNA pool. By genotyping the F2 population using these polymorphic markers, we got an initial genetic map which showed that these SSR markers were completely associated with oat dwarfing gene DwWA, and associated with each other as well, and the dwarfing gene DwWA was bracketed by the two SSR markers loci AME013 and AME117 at map distances of 13.4 and 19.9 cM, respectively.3. A new PCR-based InDel marker named bi17 was developed using the sequence information of aco245 which was closely linked to another oat dwarfing geng Dm'6. The result obtained from genotyping the parents all the F2 populationm showed that the new marker bi17 was closely linked to the oat dwarfing gene DwWA, the genetic distance between them was 1.2cM, and the log likelihood of the odds ?LOD? score between them was 121.18.4. The parents of mapping population dealed with gibberellin revealed that the dwarfing gene DwWA was GA-sensitive type; The kind of base of the SNP marker GMI_ES_LB₁1151 in the mapping population of this study was "G"; The marker bi17 was used to amplify some individuals of the NIL generated for Dw6 and the result showed that same fragment was amplified in WAOAT2132, the dwarf individual DNA pool, OT207, OT257, Potoroo and all of the dwarf individuals of the Dw6 NIL; By genotyping the progenies of the Dal*Exeter population, we got the result that the marker bi17 was linked to the SNP markers which were chosed from oat chromosome 18D, and it also indicated that the bi17 and the Mrg-04 are at the same location on the chromosome 18D.