| Mungbean(Vigna radiata L)is a kind of legume crop with strong resistance and wide adaptability,which play an important role in agricultural planting structure adjustment,high efficiency agricultural production and economic development.Bruchid is the most important pest that harms the planting,production and storage of mungbean.The screening of bruchid-resistant materials and their application in the breeding of mungbean are of great importance to the production and storage of mungbean.DNA molecular markers is widely used in crop genetic breeding,gene fine localization,germplasm resources identification and so on.With the rapidly development of high-throughput sequencing technology and the completion of mungbean genome sequencing,it provides data reference and basis for the development of mungbean InDel markers.At present,there are very few InDel markers available on the genome of mungbean and it is of great significance to develop a large number of polymorphic InDel markers for the study of mungbean correlation.The main contents of this study are as follows:1.The screening of 100 representative mungbean test bruchid-resistant was carried out.According to the degree of harm of bruchid to mungbean,the results were as follows:only one mungbean accession was screened out for bruchid-resistant.among which the resistance levels of mungbean accessions was V2709.There are 7 mungbean accessions of middle resistance including:G25,V4718,SK-2,SK-3,SK-4,ZL-3 and JL-7.SK-2,SK-3,SK-4,ZL-3 and JL-7 are proved to be resistant and adaptable by multi-point identification test,which can provide a field basis for the promotion and production of varieties.The results showed that SK-3 with high yield and resistance of mungbean after harvest.The above studies not only provide the resistant parent for the subsequent breeding of bruchid-resistant,but also provide experimental basis in production.2.The original sequence was obtained by the second generation sequencing technology,the resequencing was carried out bruchid-resistant mungbean accession V2709 and the susceptible cultivar SL-1.1809 InDel markers were generated based on the mInDel software.1809 InDel markers sequence were aligned with mungbean reference genome,1107 InDel markers were matched on chromosome and the InDel marker were sclarifies the physical location of each in chromosome,provides the basis for the gene location of mungbean research in the future.The degree of molecular marker polymorphism is an important condition for whether it can be widely used in the study.Polymorphism detection was carried out for the matching 1107 InDel markers,of which 91.9%had polymorphism.The InDel markers developed by mInDel software have been accurately evaluated and verified in the size of InDel,the distribution of chromosomes and the polymorphism of sequencing samples.3.In the test,20 representative and widely sourced mungbean varieties were selected for the screening of InDel markers,and 22 core InDel markers with full amplification and high polymorphism were obtained.Using UPGMA to construct the clustering tree of 104 mungbean varieties and the result showed that 104 mungbean varieties could be divided into 2 groups while the genetic similarity coefficient is 0.54.Genetic analysis showed that the germplasm resources of the domestic mungbean have been isolated from the effect of regional isolation.The DNA molecular fingerprint database constructed by 22 InDel markers on 104 mungbean varieties can completely distinguish mungbean varieties and provide scientific basis for the protection of mungbean varieties and the management of origin traceability. |
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