Study On The Microbial Phospholipid Fatty Acids In The Reclaimed Soil Of Different Years By Application Of The Different Kinds Of Fertilizer

In this paper, phospholipid fatty acids (PLFA) technology as the starting point, through different fertilization measures, to study the total amount of soil PLFA, the amount of bacterial PLFA, the amount of fungi PLFA,the ratio of bacterial PLFA/fungal PLFA, the amount of Gram-positive bacteria (G+) PLFA,the amount of Gram-negative bacteria (G-) PLFA,the amount of microbial biomass C and the amount of microbial biomass N on the basis of the field experiments about the different reclamation years. The conclusions as follows:(1) The different fertilization treatments in three growth stages of the com, we find that the total PLFA on the stage of heading> the total PLFA on the stage of maturity> the total PLFA on the stage of jointing. The mixing use of organic fertilizer and phosphorus fertilizer makes the total PLFA than the soil without treatment increased the most on the sage of heading. In addition, the mixing use of organic fertilizer and Phosphorus Fertilizer about the first and the third reclamation year can be increased by 372.9% and 633.9%; meanwhile which improved 48.8% and 50% with the inorganic fertilizer. Compared with the mixing use of inorganic fertilizer and phosphorus Fertilizer, the best fertilization treatment increased 33.7% and 34.3%.The seventh reclamation year's total PLFA more 136.7%-150.7% than the first reclamation year's total PLFA. With the increase of the reclamation year, the total amount of cultivated soil's PLFA is also increasing. And the highest soil's PLFA is under the mixing use of the three Fertilizers.(2) The different fertilization treatments make the soil's bacteria PLFA larger than the soil without fertilization in the corn's every growth stages. The mixing use of organic fertilizer and phosphorus fertilizer can reach the highest level in the first and the third reclamation year. It is achieve respectively 208.39ng/ kg and 366.71ng/kg, at the same time, it's higher than other treatments. The seventh reclamation year's bacteria PLFA with different fertilization treatments is more 281.6%-376.6% than the first reclamation year's and 71.5%-235.5% than the third reclamation year's. With the increasing reclamation years, the amount of soil's bacteria PLFA is also increasing. The best fertilization treatment is the mixing use of the organic fertilizer and phosphorus fertilizer, which can make the soil's bacteria PLFA reach the largest level.(3) The different fertilization treatments make the soil's fungi PLFA larger than the soil without fertilization in the corn's every growth stages. The mixing use of organic fertilizer and phosphorus Fertilizer can reach the highest level in the first and the third reclamation year. It is achieve respectively 104.86ng/kg and 204.78ng/kg, at the same time, it's higher than other treatments. The seventh reclamation year's bacteria PLFA with the different fertilization treatments, which is more 316.5% -482% than the first reclamation year's. and the number achieve 394.22ng/kg under the mixing use of the three fertilizers, so the mixing use of inorganic fertilizer, organic fertilizer and phosphorus fertilizer have a positive effect on the soit's fungi PLFA.(4) The research about the Gram-positive bacteria (G+) PLFA and the Gram-negative bacteria (G-) PLFA with the seven fertilization treatments on the different reclamation year's soil, we find that it's variety accompany with the adjunction of the different fertilizers. The seventh reclamation year's Gram-positive bacteria (G+) PLFA with different fertilization treatments is more 26.1%-26.6% than the first reclamation year's and 9.6%-14.4% than the third reclamation year's. In addition, the mixing use of the organic fertilizer and phosphorus fertilizer reached the maximum number. It is 56.27ng/kg. The most significant increase is the first and the third reclamation year's Gram-positive bacteria (G+) PLFA with the mixing use of organic fertilizer and phosphorus fertilizer, which reached 15.01ng/kg. The seventh reclamation year's Gram-negative bacteria (G-) PLFA with different fertilization treatments is more 20.2%-38.9% than the first reclamation year's and 14.1%-14.7% than the third reclamation year's. the mixing use of the organic fertilizer and phosphorus fertilizer reached the maximum number. It is 90.66ng/kg. Compared to first reclamation year's Gram-negative bacteria (G-) PLFA, the third reclamation year's Gram-negative bacteria (G-) PLFA have an obvious increase under the three different fertilizers, it's can be reached 28.74ng/kg.(5) Fertilization treatments can increase the soil's microbial biomass carbon and nitrogen. Meanwhile, it is increasing accompany with the reclamation years. The different fertilization treatments make the soil's microbial biomass carbon and nitrogen obviously larger than the soil without fertilization in the corn's every growth stages. The mixing use of organic fertilizer and phosphorus Fertilizer can make the soil's microbial biomass carbon and nitrogen reach the highest level, so it is the best fertilization treatments in this test, the soil's largest microbial biomass carbon in the first, third and the seventh reclamation year is 150.67mg/kg,184.54mg/kg and 260.21mg/kg, the seventh reclamation year's microbial biomass carbon is respectively more 72.7% and 41% than the first and the third reclamation year's. However, the soil's microbial biomass nitrogen in the seventh reclamation year is improved 81.4% and 11.1% than the first and the third reclamation year's.