Effects Of Amino Acid Manganese On Rumen Fermentation,Growth Performance And Semen Quality Of Sheep

The objective of this study was to investigate the effect of supplementation of difference levels of manganese amino acid on rumen fermentation, growth performance, the indices of blood physiological and biochemical, and semen quality in ram. Tewele wethers with permanent rumen fistulated were selected (average weight 40±1.25 kg,12 months old), and randomly divided into four groups. Based on the nutrition requirement of ram (NRC, 2007), ration of the diet was designed with supplementation with 0 (control),245,485 and 735 mg/kg amino acid manganese. The results showed that:?The rumenal pH in 485 mg/kg and 735 mg/kg groups were significantly lower (P<0.05) after feeding 3 h. The total acid concentration in 485 mg/kg group were significantly increased (P<0.05) after feeding 3 h and 6 h. The concentration of propionic acid in the 485 mg/kg group was significantly increased (P<0.05)after feeding 3 h, compared with control group.?Millet straw DM digestibility in 485 mg/kg group was significantly higher than that in the control group(P<0.05) after feeding 12 h,36 h,48 h, millet straw NDF digestibility was significantly higher than that in the control group(P<0.05) after feeding 24 h,36 h,48 h,72 h, ADF digestibility of millet straw was significantly higher than that in the control group(P<0.05) after feeding 12 h,24 h, 48 h,72 h. The effective degradation rate of DM in 485 mg/kg group was significantly higher than that in the control group (P<0.05). NDF digestion of maize straw in 245 mg/kg and 485 mg/kg groups was significantly higher than that in the control group, the same to the effective degradation rate.DM digestibility of alfalfa hay had no significant difference between groups in every time points(P>0.05), NDF digestibility of alfalfa hay in 485 mg/kg group was significantly higher than that in the control group (P<0.05) after feeding 12 h,24 h,36 h,48 h, Alfalfa ADF digestibility in 485 mg/kg group and 735 mg/kg group were significantly higher than that in the control group after feeding 36 h. The apparent digestibility of DM, CP, NDF and CF treatment group were significantly higher than those in the control group, apparent digestibility of ADF in 245 mg/kg group was significantly higher than that in the control group. There is no difference between other treatment groups and control group.Twenty five-month-old DorperxSmall Tail Han ram with weight of 33.49±1.48 kg were selected and randomly divided into 4 groups, fed respectively with supplementational 0,245, 485 and 735 mg/kg amino acid manganese in diet. Results showed that:there was no significant difference in DM feed intake between the groups. The average daily gain and the feed utilization rate of 735 mg/kg group was significantly higher than that in the control group, and there was no significant difference between the treatment groups. Activity of T-AOC, GSH-Px, SOD and serum testosterone contentin in 485 mg/kg group was significantly increased (P<0.05).The content of MDA in 485 mg/kg group was reduced, compared with the control group.Sperm concentration and the sperm motility in 485 mg/kg group was significantly increased, whereas sperm DNA fragmentation rate was decreased (P<0.05), compared with the control group. In conclusion, supplementation of the optimal level in diet was 485 mg/kg.