Exogenous Ascorbic Acid And Glutathione Daub On Tobacco After Topping Regulate And Control Active Oxygen Free Radicals And Nicotine Research

The appearance quality of tobacco in some tobacco area of China is close to the international level, but the chemical composition of tobacco leaves is inharmonious, and one of the main reason is the nicotine content is high. So do research on how to reduce the level of nicotine in tobacco is the key to tobacco research at the present stage. Topping is the reason that stimulating the nicotine content increased, the nicotine content of tobacco is low before topping, and the nicotine content rise sharply after topping. This test is from the perspective of tobacco topping, it have researched the different topping treatment that influence on the content of active oxygen free radicals, jasmonic acid and nicotine, the main results were as follows:Daub 1% AsA and daub 10% AsA after topping by iron blade, it can effectively promote the content of hydrogen peroxide, hydroxyl free radical and jasmonic acid increased, and eventually promote the nicotine content rise; Daub 1% AsA and daub 10% AsA after topping by manual operation, it can effectively inhibit the content of hydrogen peroxide, hydroxyl free radical and jasmonic acid increased, and eventually inhibit the nicotine content rise. The concentration of AsA have no effect to the content of hydrogen peroxide, hydroxyl free radical, jasmonic acid and nicotine, and buffer also have no effect. Each index rendering the response of the trend in multi-topping is related to the intensity.Daub 1% AsA and daub 1% AsA plus 1% GSH after topping by manual operation, within 48h, the super oxygen anion, hydrogen peroxide, malondialdehyde present a first rise then fall. Based on superoxide anion and hydrogen peroxide, malondialdehyde, jasmonic acid, nicotine strength of multi-topping response after data processing, we have found after daub 1% AsA and daub 1% AsA plus 1% GSH the fluctuation of ultra oxygen anion, hydrogen peroxide, malondialdehyde, jasmonic acid and nicotinamide is less than conventional topping and daub buffer after topping. Among them,the treatment that daub 1% AsA plus 1% GSH after topping have the strongest inhibitory effect to active oxygen free radicals, jasmonic acid and nicotine.In the field, we adopt a different approach, such as top-remaining, conventional topping, daub 1% AsA,1% AsA plus 1% GSH and buffer after topping, the experimental results showed that in the treatment of top-remaining, the difference of nicotine content between upper leaves and middle leaves is small, but the nicotine content in upper leaves and middle leaves is significantly higher than the lower leaves. In the other treatments, the nicotine content in different parts is upper leaf> middle leaf> lower leaf. The treatment that daub 1%AsA or 1%AsA plus 1% GSH after topping did not change the distribution of nicotine in the tobacco, but the nicotine content in different parts were significantly lower than the treatment of conventional topping and the treatment that daub buffer solution after topping, the nicotine content in the lower, middle, upper and top leaves of the treatment with daub 1% AsA (1.74%,2.46%,2.98%,2.46%) and the nicotine content in the lower, middle, upper and top leaves of the treatment with daub 1% AsA plus 1% GSH (1.85%,2.35%, 2.87%,2.35%) were significantly lower than the nicotine content in the lower, middle, upper and top leaves of the treatment with conventional topping (2.42%,2.51%,3.38%, 2.51%).The difference in the field between daub 1%AsA and daub 1%AsA plus 1%GSH is no significant. daub 1%AsA and daub 1%AsA plus 1%GSH after topping have a certain resistance to brown spot, the incidence of brown spot were 29.1% and 31.4%, respectively, and the disease index of brown spot were 9.2 and 10.1 respectively, they were significantly lower than the conventional topping, in the treatment of conventional topping, the incidence and disease index of brown spot were 44.2% and 14.9,respectively.The yield of daub 1% AsA, daub 1% AsA plus 1% GSH, and conventional topping were 147.44 kg/667 m2,144.8 kg/667 m, and 142.9 kg/m2,respectively, and they didn't have too big difference, but the proportion of mid high grade leaves, the average price and the production value with daub 1%AsA and daub 1%AsA plus 1%GSH were both promoted significantly than with the conventional topping, the proportion of mid high grade leaves with daub 1%AsA, daub 1%AsA plus 1%GSH,and conventional topping were 82.2%,83.3%, and 75.1%, respectively, the average price were 20.24 yuan/kg,21.53 yuan/kg, and 19.02 yuan/kg, respectively, the production value were 2985.40 yuan/667 m2,3117.54 yuan/667 m2, and 2717.96 yuan/667 m2, respectively.