| In this paper,experiments were conducted to systematically evaluate the effects of dietary methionine,bile acid,taurine and Non-Starch Polysaccharide Enzyme supplementation in low fishmeal based diets on the growth performance,body composition,feed utilization,digestive enzyme activity and antioxidant ability of juvenile Chinese mitten crab,Eriocheir sinensis.The results were followed.Effects of dietary methionine or bile acid or taurine supplementation in Low fishmeal diet on growth performance,feed utilization and antioxidant capacity of juvenile Chinese mitten crab(Eriocheir sinensis)Abstract:Experiments were conducted to evaluate the effects of dietary bile acid,taurine and methionine supplementation in low fishmeal based diets on the growth performance,feed utilization and antioxidant ability of juvenile Chinese mitten crab,Eriocheir sinensis.Five diets were formulated using a basic diet,in which 15%fish meal was included,supplemented with 0.5%methionine,0.5%?1.0%bile acid,0.5%?1.0%taurine,respectively.Diets of either full fishmeal-based(fish meal accounted for 64.4%)or low fishmeal-based(fish meal accounted for 15%)served as the controls.Each diet was fed to juvenile Eriocheir sinensis[(0.26±0.02)g]with five replicates for 8 weeks.The results showed that,compared with LFM diet,weight gain,specific growth rate of crabs fed FM and 0.5%Met diets were significantly higher(P<0.05),meanwhile the feed coefficient of crabs fed FM and all treatment groups exhibited significantly lower values(P<0.05).Crabs fed FM has the highest protein efficiency,and no significant differences were found among this group and the groups of 0.5%Met,1.0%Bile,0.5%Tau and 1.0%Tau.Whole body proximate composition of E.sinensis fed different experimental diets has no significant differences,only the lipid content of crabs fed o.5%Met was higher than group of 1.0%Bile and 1.0%Tau.Crabs fed LFM had the highest amylase activities in the intestine and hepatopancras,and in contrast crabs fed FM obtained the lowest values.Crabs fed FM and 0.5%Met exhibited the highest total protease activity in crab intestine followed by crabs fed 0.5%Bile diet.Both in intestinal tract and hepatopancreas,Crabs fed 0.5%Met diet obtained the highest intestine and hepatopancreas lipase activities.Crabs fed 1.0%Tau exhibited the lowest lipase activity in hepatopancreas.Crabs fed LFM had the lowest lipase activity in intestine.Malondialdehyde(MDA)content in crabs fed LFM was significantly higher than crabs fed FM and other diets.Crabs fed FM diet had significantly higher activities of superoxide dismutase(SOD)and catalase(CAT)than fed other diets.The crude lipid content of carbs fed 0.5%Met was the highest and significantly higher than group of 0.1%Bile and 1.0%Tau.All these results suggest that supplementation of appropriate levels of bile acid and taurine could effectively reduce feed coefficient,and dietary 0.5%Met supplementation could effectively improve the growth performance,activities of total hepatopancreas or intestine protease and lipase,and oxidation resistance.Dietary 0.5%,1.0%bile acid supplementation could also effectively improve the activities of total protease and lipase,but no differences were found in accelerating the growth performance.Besides,Dietary 0.5%,1.0%Taurine supplementation could effectively improve activities of total hepatopancreas or intestinal protease and lipase and oxidation resistance,but cannot accelerate the growth performance of E.sinensisEffects of Non-Starch Polysaccharide Enzyme supplementation in Low fishmeal diet on growth performance,feed utilization and digestive enzyme activities of juvenile Chinese mitten crab(Eriocheir sinensis)Abstract:Experiments were conducted to evaluate the effects of Non-Starch Polysaccharide Enzyme supplementation in low fishmeal based diets on the growth performance,feed utilization and digestive enzyme activities of juvenile Chinese mitten crab,Eriocheir sinensis.Five diets were formulated using a basic diet,in which 15%fish meal was included,supplemented with 0.5%micro encapsulated coating amino acid including methionine and lysine,and another kind of non-starch polysaccharide complex enzyme including xylanase,pectinase,?-glucanase and cellulose which concentration is 1250U/kg fodder,respectively.Diets of either full fishmeal-based(fish meal accounted for 64.4%)or low fishmeal-based(fish meal accounted for 15%)served as the controls.(Denoted as +AA,+ enzyme,+1+both,FM,LFM)Each diet was fed to juvenile Eriocheir sinensis[(0.14 ±0.01)g]with five replicates for 8 weeks.The results showed that,compared with LFM and FM diet,there are no significant differences in weight gain,specific growth rate of crabs,feed coefficient and hepatopancreas index of all treatment groups.Compared with LFM diet,the protein and lipid content of crabs fed with FM,and complex enzyme were significantly higher than group of LFM.The total hepatopancreas protease activities of crabs fed coating amino acid and complex enzyme were higher than groups of LFM,and than groups of FM and +both has the total hepatopancreas protease activities.The total hepatopancreas lipase activities of treatments were significantly higher than group of LFM,but no significant difference with group of FM.In the intestinal tract,compared with group of LFM,the total protease activities of all treatments showed significantly higher.Carbs fed with FM and both of coating amino acid and non-starch polysaccharide complex enzyme had significantly higher total hepatopancreas lipase activities in the intestinal tract than group of LFM.All these results suggest that supplementation of coating amino acid and non-starch polysaccharide complex enzyme could effectively improve crude protein and lipid contents.In the meanwhile,adding both of them could effectively improve total intestinal lipase vigor.Effects of adding different concentration of Non-Starch Polysaccharide Enzyme supplementation in Low fishmeal diet on growth performance,feed utilization and digestive enzyme activities of juvenile Chinese mitten crab(Eriocheir sinensis)Abstract:Experiments were conducted to evaluate the effects of adding different concentration of Non-Starch Polysaccharide Enzyme supplementation in low fishmeal based diets on the growth performance,feed utilization and digestive enzyme activities juvenile Chinese mitten crab,Eriocheir sinensis.Five diets were formulated using a basic diet,in which 15%fish meal was included,supplemented with 0.5%micro encapsulated coating amino acid including methionine and lysine,and on these basis add different concentration of non-starch polysaccharide complex enzyme including xylanase,pectinase,P-glucanase and cellulose(0,625,1250,2500,5000U/kg).And than diets of either full fishmeal-based(fish meal accounted for 64.4%)or low fishmeal-based(fish meal accounted for 15%)served as the controls.Each diet was fed to juvenile Eriocheir sinensis[(0.14±0.01)g]with five replicates for 8 weeks.The results showed that,there are no significant differences in weight gain,specific growth rate of crabs,feed coefficient,protein efficiency ratio and hepatopancreas index among all groups.The same as the protein,lipid and ash content of crabs,they also showed no significant differences,but the lipid of group of +5000 was higher than others.The total hepatopancreas protease activities of crabs fed complex enzyme which concentrations were 1250 and 2500U/kg were significantly higher than other groups,and the intestinal lipase activities of crabs fed complex enzyme which concentrations were 2500 and 5000U/kg were significantly higher than other groups.All these results suggest that supplementation of non-starch polysaccharide complex enzyme which concentrations were 1250 and 2500U/kg could effectively improve the total hepatopancreas protease.In the meanwhile,adding complex enzyme which concentrations were 2500 and 5000U/kg could effectively improve total intestinal lipase vigor and when adding complex enzyme which concentrations were 5000U/kg the lipid content were effectively improved. |
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