| Daheng broilers is a new national independent cultivation of broiler. This experiments were conducted to study the appropriate amount of feed by force-feeding in TME assay with Daheng broilers, to evaluate the nutritional value of soybean meal (SBM) and rapeseed meal (RSM) from different origins, and to research the additivity of this two kinds feedstuffs with corn. The objective was to provide the basic feed nutritive value data for Daheng Broiler feedstuff database.Exp.1. Study on the appropriate amount of feed by force-feeding in TME assay in Daheng broilers. A total of 40 (18-week-old) male Daheng broilers were completely randomized design divided into 5 groups with 8 replicates of 1 chicken. These groups were force-fed amount of 0,1.0,1.5,2.0, and 2.5% of body weight feed, while 0% group was used as negative controls for the measurement of endogenous losses of metabolizable energy.TME assay was used in experiments, with 48h starvation and excreta was then collected for 48h. The results showed that:the different force-feed levels had no significant effect on body weight loss, total nitrogen retained, and energy utilization (P> 0.05). The average AME, AMEn, TME, and TMEn in corn-soybean diet were 2.768,2.868,3.204, and 3.307 kcal/g, respectively. There were no significant differences for AME and AMEn but significant differences (P< 0.05) for TME and TMEn among treatments. The CV of 2.0%treatment is lower than that of 1.5%. In conclusion, considering the weight loss and nitrogen retention, the 2.0% force-feed level is appropriate for Daheng broilers.Exp.2. Metabolizable energy and true amino acid availability of soybean meal in Chinese Daheng broilers.12 SBMs were collected from representative feed company in Sichuan province.100 conventional prepared for the metabolisable energy and while 100 cecectomized broilers were for TAAA study. The metabolic energy evaluation, divided into 3 times and 48 conventional Daheng broilers were randomly assigned to 6 treatments with 8 replicates of 1 chicken in each time. The TAAA evaluation were divided into 4 times,36 cecectomized Daheng broilers were randomly assigned to 6 treatments with 6 replicates of 1 chicken in each time. The semi-purified diets containing 17% protein by formulated SBM with corn starch. The results showed that:DM, CP, GE, NDF, ADF, CF, EE, and Ash were 85.74%,52.81%,4.73kcal/g,13.61%,6.47%,6.67%,1.52%, and 6.63%, respectively. The CV of NDF, CF, and EE were greater than 15%. The amino acid content and CV of SBMs were ranged from 0.56 to 7.99% and from 6.36 to 10.94%. The content and CV of indispensable, dispensable, and total AA were 19.26% and 7.35%,24.66% and 7.10%, 43.92% and 7.18%, respectively. The maximum CVwas 10.94% in Pro. The AME, AMEn, TME, and TMEn of the twelve SBM samples were 2.993,3.091,3.058 and 3.122kcal/g, respectively. Significant differences (P< 0.05) were observed in metabolic energy of different sources SBM. The average of different kinds of AA were 78.16?94.38% and there were significant differences (P<0.05) among the sources. The average of indispensable, dispensable, and total AA were 84.32%,82.29%, and 82.88%, respectively.Exp.3. Metabolizable energy and true amino acid availability of rapeseed meal in Chinese Daheng broilers.12 RSMs were collected from representative feed company in Sichuan province for the metabolic energy and True amino acid availability study. The metabolic energy evaluation were divided into 3 times,48 conventional Daheng broilers were randomly assigned to 6 treatments with 8 replicates of 1 chicken in each time. The TAAA evaluation were divided into 4 times,36 cecectomized Daheng broilers were randomly assigned to 6 treatments with 6 replicates of 1 chicken in each time. The results showed that:DM, CP, GE, NDF, ADF, CF, EE, and Ash were 87.67%,42.10%,4.72kcal/g, 39.99%,16.13%,15.15%,2.40%, and 9.10%, respectively. The CV of NDF, ADF, EE, and Ash were greater than 15%. The amino acid content and CV of RSMs were ranged from 0.72 to 6.55% and from 4.53 to 18.97%. The content and CV of indispensable, dispensable, and total AA were 14.39% and 7.25%,19.02% and 5.85%,33.42% and 6.25%, respectively. The maximum CVwas 18.97% in Lys. The AME, AMEn, TME, and TMEn of the twelve RSMs were 2.062,2.158,2.229, and 2.323kcal/g, respectively. Significant differences (P< 0.05) were observed in metabolic energy of different sources RSM. The average of different kinds of AA were 68.37 to 88.92% and Except Thr and Ser, the significant differences (P< 0.05) of TAAA among the other AA. The average of indispensable, dispensable, and total AA were 70.81%,70.73%, and 70.45%, respectively.Exp.4. Additivity of values for metabolizable energy and true amino acid availability in SBM/RSM/corn fed to Daheng broilers:This experiment was conducted to d verify the accuracy of the determined TMEn and TAAA value in SBM and RSM Chinese Daheng broilers. One corn, several SBM or RSM were random selected and formulated for three diets:corn-SBM diet, corn-RSM diet, and corn-SBM-RSM diet. AME, AMEn, TME TMEn, and TAAA were measured. For the metabolic energy evaluation, divided into 3 batches,48 conventional Daheng broilers were randomly assigned to 6 treatments with 8 replicates of 1 chicken in each batch. For the AA evaluation, divided into 4 batches,36 cecectomized Daheng broilers were randomly assigned to 6 treatments with 6 replicates of 1 chicken in each batch. The results showed that:There were no differences between the predicted and the measured values of TMEn (P>0.05), While except His, Met and Cys in TAAA (P> 0.05).In conclusion, the 2.0% force-feed level was used to determin the metabolizable energy and true amino acid utilization of different sources of 12 soybean and rapeseed meals, there were significant differences (P< 0.05) of the ME and TAAA among different sources in SBMs and RSMs. There were no differences between the predicted and the measured values of TMEn and true amino acid availability except for histidine, methionine and cysteine, which showed the additivity in TMEn and TAAA. |
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