The Comprehensive Mechanisms Underlying Nonhierarchical Follicular Development In Geese And Preliminary Exploration Of Gene Function Of AKR1D1

The growth and development of follicular directly affects the performance of laying goose, and the underlying nonhierarchical follicular development in geese has two special stages:follicle recruiting stage (small white follicles,SWF,2-4mm) and the dominant follicle screening stage (small yellow follicles,SYF,8-10mm).These two different stages of follicle selection have different results, the follicle recruiting stage get a lot of small follicles continue to develop,and the dominant follicle screening stage only get a follicle continues into hierarchical follicular development until ovulation. In order to explore the different of molecular mechanisms network in the two stages, this experiment respectively employde two stages of SWF and SYF of nonhierarchical follicular development in geese as experimental materials,the Illumina/Solexa sequencing and digital gene expression spectrum (DGE) were uesd to screened and identified the differentially expressed genes between SWF and SYF, then the GO and KEGG pathway related to DEG were also analyzed.The results showed that AKR1D1 expressed in small white follicles and small yellow follicles, had a significantly different expression levels between SWF and SYF. The main biological function of AKR1D1 is the synthesis of bile acids and steroid hormones cleared,and the key of normal follicles development is steroid hormone homeostasis,so we will preliminary exploration of gene function of AKR1D1 in follicular development.The main findings are as follows:1. Construction of SWF and SWF library were detected 1.59G and 1.71G data through a series quality control after filtration to give higher credibility 1.56G and 1.68G of Clean sequence.2.Using the software of RSEM,we analyzed the gene mapping of two groups respectivel y, positioning the two groups resulting percentages were 88.80% and 89.21%, higher than 70%, indicating that the results are more ideal.3. A total of 160 differentially expressed genes were screened through DGE and 114 genes got positively regulated,46 genes got negatively regulated among them.4. In this study, by differentially expressed genes were enriched GO and KEGG Pathway, enriched to a lot of SWF and can be interpreted in two stages SYF follicle development path difference, such as cell proliferation, cell differentiation, nutrient supply transport, energy, amino acid metabolism and lipid metabolism pathway, which you can deduce the steroid synthesis, cell proliferation and cell differentiation play a significant role in the SWF follicular development stage.5. This study get goose AKR1D1 gene CDS sequences 981bp, it encoding 326 amino acids. AKR1D1 gene amino acid of geese and chickens, turkeys similarity were higher than 90%; the AKR1D1 protein localized of goose in the cytoplasm and mitochondria, are not secreted protein, the presence of 18 potential phosphorylation sites and 3 glycosylation sites.And the protein tertiary structure of a curved spiral; qRT-PCR showed in granulosa layer and the film of 2-4mm have highest expression, was the lowest in the granular layer film of F5 and F1.