| The experiment aims to understand the feasibility with respect to Paramisgurnus dabryanus dietary fishmeal substitution with earthworm meal and its optimal level of replacement. A total of 390 active fish, with an average initial body weight of 3.86±0.23g, was allocated into 5 groups (groups A to E), which has 3 replications comprising 26 fish per replication. The five levels[0%(A),25%(B), 50%(C),75%(D),100%(E)] of Dietary fishmeal substitution with earthworm meal formulated to 5 experimental feeds were fed to fish for 10 weeks. After fasting for 24 hours, every single replication randomly selected 12 fish were prepared for experimental samples, which is blooded caudal vein to make serum and then take out hepatopancreas and spleens to make tissue homogenate and also isolate muscle to use. Growth performance, serum biochemistry, digestive enzyme and immunity performance in loach Paramisgurnus dabryanus and its filial generation were observed. At the end of the experiment, each group randomly selected 10 fish were met challenge bacterium 1.62×109CFU/mL of Aeromonas hydrophila (median lethal dose), which employs intraperitoneal injection using 0.2ml for per fish. It lasted 15 days to understand the different level of dietary earthworm meal to response to experimental fish infected disease. There is a majority of results as follow:1 Effects of subsititution fishmeal with earthworm meal on growth in Paramisgurnus dabryanusThe differences of survival rate (B to D), gain weight rate (B to D), condition factor(B to E), food coefficient(B to E), specific growth rate(B to D) between the control group and Administred group were not significant (P>0.05), however, survival rate of group E was significantly lower than that of the control group (P<0.01).2 effects of substitution fishmeal with earthworm on body composition Paramisgurnus dabryanusThere were no significant differences between treatment groups (B to E) and the control group as to the contents of crude fat, moisture and driage. The contents of crude protein in muscle of all experimental groups was predominantly lower than that of the control group (P<0.01).3 effects of subsititution fishmeal with earthworm meal on serum biochemistry in Paramisgurnus dabryanusOn one hand, compared with the control group, the activity of ALT in serum of groups C were no significant differences(P>0.05). On the other hand, the activity of ALT in serum of group B, group D and group E were outstandingly higher as compared to the control group(P<0.01). However, the activity of AST in serum of treatment group was obviously lower than that of the control group (P<0.01).4 effects of substitution fishmeal with earthworm meal on blood lipid in Paramisgurnus dabryanusThere were no significant differences between the contents of TG of group B, and of HDL-C of groups B to E, where the contents of TG of groups C to E were significantly higher than that of the other groups(P<0.01). the contents of T-CHO of groups D to E were obviously higher than that of other groups (P<0.01), here the contents of T-CHO of group E was significantly higher than that of the other groups(P<0.01).5 effects of substitution fishmeal with earthworm meal on digestive function in Paramisgurnus dabryanusThe activity of intestinal AMS of group C, of intestinal LPS of groups B to D, were not significant compared to the control group(P>0.05). The same tendency was also detected in the height of intestinal villus of groups B to D, and in the hepatopancreas index of groups B to E(P>0.05). However, the activity of intestinal AMS of group B and groups D to E were predominantly higher compared with the control group(P<0.01).6 effects of substitution fishmeal with earthworm meal on antioxidant function in Paramisgurnus dabryanusThe activity of T-AOC of groups B to E were no outstanding differences compared to the control group(P>0.05), where was predominantly higher than in the activity of CAT(groups B to E), and in the contents of MDA(group B and groups D to E)(P<0.05), However, where was obviously lower than in the the activity of T-SOD (groups B to E) and GSH-PX(group E) (P<0.05).7 effects of substitution fishmeal with earthworm meal on immunity function and protection from infected Bacteria Aeromonas hydrophilia in Paramisgurnus dabryanusThe differences of spleen index(group B, group D), and of activity of AKP and contents of LZM in hepatopancreas(groups B to D), and of of morbidity (groups B to C) and survival rate (group C) were not significant compared to the control group(P >0.05). however, the spleen index of group B, group D and the control group was all significantly higher (P<0.01), the activity of AKP in hepatopancreas of group E was notably higher(P>0.05), morbidity of groups D to E were remarkably higher(P> 0.05), survival rate of groups D to E were outstandingly lower(P>0.05), as compared with that of the control group.Under laboratory condition, we discover that 25% of dietary fishmeal replacement by earthworm meal could not significantly affect production performance and immunity respond of Paramisgurnus dabryanus. Consequently for those agents including grwoth performance and premunition and muscle compositions, we suggest that 39.11%% of dietary fishmeal of substitution with earthworm meal is very potent. Meanwhile we advise that 35.43% of dietary fishmeal can be alternated with earthworm meal while implementing the estimation of growth and immunity performance. |
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