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Creeping Chrysanthemum Cross Breeding And Genetic Research Offspring

Posted on:2016-08-16Degree:MasterType:Thesis
Country:ChinaCandidate:F CuiFull Text:PDF
GTID:2323330512960952Subject:Garden Plants and Ornamental Horticulture
Abstract/Summary:PDF Full Text Request
This test first around collected from different disc type, pattern and color of chrysanthemum cultivars, and to choose 10 cultivars and advantageous traits a creeping varieties, of different hybrid combination, respectively, in 2012,2013 and July 2014-October artificial hybridization experiment was carried out. and get a batch of hybrid progeny. To observe some phenotypic traits of hybrid progeny records and analysis. The results showed that:1. The statistical analysis of prostrate by chrysanthemum creep properties of hybrid offspring:erect, creeping properties in characterized in population of hybrid offspring, erect growth account for 72.8%, Creeping growth 21.8% and Umbrella growth 5.4%, prostrate growth plants and upright growth of plants is 3:1. The separation consistent with Mendel rule. Umbrella plant and hybrids appeared, upright and creep character is controlled by a pair of incomplete dominance main gene.2. Chrysanthemum in the aspect of quality character (creep properties except) mainly includes:double sex. design and color, etc.. the author carried out a statistical analysis on the double sex character of hybrid offspring based on experiments, and found the chrysanthemum genetic obvious double sex. F1 generation more than double sex between male and female parent. Through the analysis and design and color is light pink, pink and gold, the highest proportion of tan. chrysanthemum flower color with partial maternal genetic.3. Further discussion from the molecular level, according to the genetic diversity of chrysanthemum resources. This experiment with 20 for chrysanthemum SSR primers for selected 33 chrysanthemum seed and the parents were analyzed, a total of 53 allelic variation is detected. By population structure analysis, principal component analysis, clustering analysis, three methods, never tongfang face clustering chrysanthemum varieties, are the chrysanthemum is divided into two categories, one group with kingdee play pearl×the red carpet for their offspring have 10 strains, accounting for 71.4% of the first groups;× kingdee to red carpet show bead hybrids with 4 strains, accounting for 28.6% of the first groups, show the maternal inheritance. The second group, on the red carpet × kingdee drama beads as three strains of hybrid progeny, accounted for 42.9% of the second groups; In kingdee play pearl×the red carpet for the hybrid offspring of four strains, accounting for 57.1% of the 2 groups.3,The genetic diversity of chrysanthemum were discussed from molecular level micro resources:this experiment design, and 20 to the chrysanthemum SSR primers, and SSR primers of 33 chrysanthemum seed and the parents were analyzed,53 allelic variation is detected. By principal component analysis, clustering analysis method for clustering chrysanthemum varieties, the chrysanthemum is divided into three categories:the first group is 'Jindiexizhux' X 'Hongditan', play them with the 'Jindiexizhux' show bead showed a closer relationship; The second group of hybrids with the characteristics of the 'Hongditan' close relatives; Third group mainly includes the 'Yulong','Yuanguanzi' and 'Zhanhuangzao' such as the male parent. The results show that the hybrid chrysanthemum show some partial maternal inheritance. SSR analysis and morphological characters of hybrid offspring performance has certain experimental data, available molecular markers for genetic and breeding research phenotypic traits have certain guiding significance.
Keywords/Search Tags:chrysanthemum, hybridization, traits and SSR
PDF Full Text Request
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