Prevalence And Analysis Of Mitochondrial Partial Genes Of Haemonchus Contortus From Liuyang Black Goats

The study is on the basis of the infection of H.contortus of Liuyang black goats.To analysis morphological and molecular biological of H.contortus.To provide a scientific basis for the prevention and treatment of sheep nematode.The first part of this study is to using the autopsy methods to investigate the infection of H.contortus in black goat of Liuyang The investigation results show that:Eastern black goat H.contortus infection rate was 75.00%, the Southern District H.contortus infection rate was 66.67%; Western H.contortus infection rate was 57.14%; the North H.contortus infection rate was 40.00%; the town H.contortus infection rate was 35.71%;Eastern H.contortus has the highest infection rate was 75.00%, town H.contortus has the lowest infection rate 35.71%.The second part of this study was studied mitochondria genes cox1, cox3 and cytb genetic variation of H.contortus of Liuyang Black Goats, From the perspective of mitochondrial genes to research each of mitochondrial genetic variation.In order to find the effective genetic marker to study molecular taxonomy and population genetic relation ships of H.contortus;then using BLAST to compara H.contortus gene Online.The findings of this study DNA fragments have been reported in GeneBank H.contortus strains AB682707.1 and EU346694.2 similarity of 97%. Finally, using Mega 4.1 software for seven H.contortus sequence analysis showed that H.contortus coxl cox3 cytb sequence similarity higher degree of conservatism in the species.The similarity rate was up 93%.