Alanalysis On Gβ-like/Cpc2,Plasma Membrane-bound H~+-Atpase MoPma2 And Transcription Factor Momybl In Pathogenicity Of Magnaporthe Oryzae

Rice blast caused serious loss of rice yield,threatening global food security.It causes by the Magnaporthe oryzae.The main prevention and control of rice blast is breeding resistant varieties,supplemented by chemical control.Because the pathogen races are volatile,resistant varieties will be loss after planted in the field for a period of time.therefore,the prevention and control of rice blast is one of the difficult problems in rice production.To understand the molecular mechanism of Magnaporthe oryzae growth and pathogenic process is an important prerequisite for the control.G protein signal transduction process plays a very important role in Magnaporthe grisea.Various aspects are involved in the regulation of rice blast disease,including growth,conidia production,appressorium formation and pathogenicity.Regulatorsors of G-rotein Signaing(RGS)is a negative regulator of G protein signaling pathway.There are 8 Rgs in Magnaporthe oryzae The MoRgs7 involved in the regulation of pathogenicity of Magnaporthe grisea.To expand our studies on regulators of G protein signaling,we constructed Co-Immune precipitation using Rgs7 as bait respectively.then mass spectrometry.were applied to analyze the composition of Protein complexes.Among the candidate proteins,We find a G protein βsubunit beta like protein MoCpc2.Further verified the interaction between MoCpc2 and MoRgs7 by yeast two hybrid assay.MoCPC2 is homologous to S.ceresiae ASCI.The results showed that the MoCPC2 gene is involved in the regulation of intracellular cAMP level.In addition A Mocpc2 mutant showed spore production significantly reduced,can not form a normal appressorium turgor,can’t successfully invade host cells leading to pathogenicity decreased significantly.At the same time,MoCPC2 gene involved in the regulation of the mycelial nutrient growth,sexual reproduction and showed low sensitivity to different stress factors and temperature.P-ATPase(plasma membrane H+-ATPase)is a kind of H+-ATPase present in the plasma membrane use the energy formed by the intracellular ATP hydrolysis to realize the reverse transmembrane proton.to generate and maintain the the electrochemical gradient of cell membrane H+,to transport of nutrients and ions.To determine the function of MoPMA2,targeted gene was replaced by gene knock-out strategy and generated a knock-out mutant△Mopma2.This article carries on the analysis of its function.The results showed that △Mopma2 loss of sporulation ability,reduced turgor pressure,decreased appressorial penetration and pathogenicity.Transcription factors(TFs),one of the largest families of trans-acting molecules,play important roles during growth,development and pathogenisis of most fungus.The Myb gene super-family comprises a group of related genes found in plant,animal,and fungal genomes.We cloned the M.oryzae MoMYB1 and characterized its role in conidiation,virulence and stress response.