Differential Gene Expression Analysis Of The Flubendiamide Insecticides In Resistant Populations Of Plutella Xylostella

Diamondback moth?Plutella xylostella L.?belongs to Lepidoptera Plutellidae causing serious damage to the cruciferous vegetables and has a high level of resistance to most kind of insecticides.With the anthranilic diamides insecticides application such as flubendiamide,result in different resistance levels.In order to study the resistance mechanism of the flubendiamide insecticides in diamondback moth,the RNA-Seq analysis method was used in this study to analysis the genetic differences between susceptible strain?S?and the two insecticide resistance strain in transcriptional level?FF28 and ZC36?.For the three strains of diamondback moth,the differences expressions of the gene were analyzed in vivo,such as biological process and pathway enrichment.Screening and bioinformatics analysis the genes related to the resistance of the diamondback moth.So as to further reveal the resistance mechanism in the direction of chemical insecticides.The main contents are as follows:1.Compared with the susceptible population S,there were 540 expression significantly different genes of the population of FF28?which raised significantly with 411,reduced significantly the 129?,and 439?299 significantly up-regulated and down was a total of 140?of ZC36.2.Statistics this part of the difference of resistance genes in biological process and pathway,we find the different genes are mainly significantly enriched in response to stimuli and chemicals,environmental pressure,metabolism,lipid and amino acid and protein metabolism,MAPK,PPAR,Wnt signaling pathway and enzyme activity regulation.3.We concluded that gene expression was upregulated mainly affects the activity of detoxification enzymes in the metabolic pathway,which had 218 significantly up-regulated intersection genes of the FF28 and ZC36 resistant populations.And only 50 was annotated into the biological process?BP?,associated with resistance way only more than 30.The 25 intersection up-regulation genes of S-VS-FF28 and ZC36-VS-FF28 were mainly enriched in "response to stimulus" "cellular protein metabolic process" "defense response" "response to stress".4.The expression of the RyR receptor: although two resistant populations showed no significant difference in expression in the ryanodine receptor,but compared with the susceptible population,change is still quite obvious.The RyR expression of field populations?ZC36?was came down.While in the FF28 population,the expression level was partly down regulated.We speculated that it might be related to the degree of heterozygosity?homozygous and heterozygous or the expression of the resistance of the hybrid?.5.We found a variety of metabolic detoxification enzymes of insects,such as cytochrome P450 superfamily,carboxylic acid esterase?CarE?,Glutathione S-transferase and heat shock proteins superfamily?HSPs?maybe involved in immune defense response.Three genes?XM₀11551310.1,XM₀11556264.1,XM₀11553349.1?involved in the "Melanogenesis" were significantly up-regulated,it maybe associated with phenolic substrate oxidation metabolism in vivo,thereby affecting insect cuticular hardening and blackening.