Bacterial Community Structure And Function In Rhizospheric Soil Of Panax Notoginseng

Notoginseng(Panax notoginseng)is an important economic and medicinal value herbal medicine,which has serious continuous cropping obstacles.The change of soil microbial community is considered as the main reason for the death of Panax notoginseng.However,the law of its changes and the mechanism of action are still unclear.Therefore,it is undoubtedly that has a great significance to systematically study the changes of microbial communities during the planting period.In this paper,the structure and function of the bacterial community were examined at the DNA and RNA levels in the different seasons,healthy and sick,plant ages of 1 year,2 years and 3 years.First,the total DNA of microorganisms in the rhizosphere soil of Panax notoginseng was extracted and sequenced in order to study the total bacterial community in the rhizospheric soil of Panax notoginseng.The results showed that the rhizosphere soil bacteria had high abundance and diversity.The diversity of the samples in the dry season is higher than that in the rainy season,and the sick samples are higher than that of health.The dominant bacteria in the rhizosphere soil of the Panax notoginseng were Proteobacteria,Bacteroidetes,Acidobacteria,Chloroflexi and Actinobacteria.A large number of Pseudomonas,Rickettsiaceae and amurskyense are enriched in the sick Panax notoginseng.In the function,differences in the sickness and health of Panax notoginseng in amino acid metabolism synthesis,tRNA synthesis process and secondary product metabolism.Secondly,total RNA was extracted from the rhizosphere soil of Panax notoginseng,and the active bacterial community was studied.The results showed that the active bacterial community was a subset of the total community,and the active bacterial communities in different samples are different,and different communities may be shaped by different environmental factors.Compared with healthy Panax notoginseng,the unique four phylums of the active bacterial community in the rhizosphere soil of the Panax notoginseng are the Cyanobacteria,Bacteroidetes,Actinobacteria and Acidobacteria.The Bacillus and Anoxiybacillus have inhibitory effects on other bacterial populations.The main environmental factors affecting the rhizosphere active bacterial community are organic>available nitrogen>available potassium>phosphorus>available phosphorus.Finally,the structure and function of the microbial community in the rhizosphere soil of Panax notoginseng were studied by metagenomics.The rhizosphere soil microbes are mainly involved in carbohydrate metabolism,amino acid metabolism,energy metabolism,nucleic acid metabolism,cofactor and vitamin synthesis metabolism,replication,recombination,membrane transport and other biochemical processes.The increase of the sickness Panax notoginseng soil microbial gene abundance in amino acid metabolism,environmental factor response and signal transduction,this leads to a greater adaptability to the environment in the rhizosphere soil microbes.The soil bacterial community of Pa,nax notoginse,ng has an important role in soil nitrogen cycling,methane metabolism and sulfur metabolism,and it's an important driving force for the chemical cycle in the rhizosphere soil.This study systematically studied the bacterial community structure and its function in the rhizosphere soil of Panax notoginseng,and the active bacterial community in the rhizosphere soil of Panax notoginseng was studied for the first time.The results showed that low of changes in the bacterial community in the rhizosphere soil of Panax notoginseng during continuous cropping.The ecological function of soil microbes was elucidated.Provides important information from the perspective of soil microecology for the continuous planting of Panax notoginseng and its disease control.