Dynamic Response Of Weat Atioxidant To Low Temperature And Differential Expression Analysis Of Related Genes

Plant growth and development are affected by many factors,such as: temperature,light,water,heavy metals,pests and diseases,which low temperature is the greatest impact on crop yield one of the adversity conditions.Heilongjiang is a cold area in northern China,the winter temperature is low and the duration is longer,winter wheat can survive in Heilongjiang Province has been breeding on a worthy of a breakthrough problem.In 2007,the Agricultural Breeding Research Center of Northeast Agricultural University successfully cultivated a new variety of Dongnong Dongmai No.1,making winter wheat cultivated in alpine region.However,the mechanism of how to accurately evaluate the effect of antioxidant level on the cold resistance of wheat has not been fully revealed.In this experiment,three winter wheat cultivars "Dongnong Dongmai 1","Ji Mai 22" and "Chnese Spring" were selected as the materials,and the field physiology test was set up at low temperature acclimation period and frozen period.The content of reactive oxygen species(SOD),antioxidant activity and antioxidant activity of three wheat cultivars were determined by simulating the low temperature environment.The dynamic changes and regulation of interstitial oxygen content in different cold cultivars were determined.mechanism.Through the comparison of indoor simulated low temperature and field real situation,it is judged whether the indoor simulated low temperature treatment can evaluate the real situation of the field and lay the foundation for the quick identification method of indoor antioxidant index.At the same time,we selected "Dongnong Dongmai 1" and "China Spring" as the experimental materials.In this study,we concluded that the winter wheat cultivars with different cold tolerance at different temperatures were used to determine the expression of winter wheat Enzyme gene,from the digital expression profile of the three superoxide dismutase-related expression genes,three ascorbate peroxidase-related expression genes,the reason why the choice of these six genes because it is detected in the The results showed that these genes were related to the cold accumulation of winter wheat during acclimation.The differences of superoxide dismutase(SOD)and ascorbate or oxidase(APX)-related genes in cold winter wheat under low temperature stress were measured by indoor low temperature simulation.Finally,through the combination of indoor and outdoor physiological and biochemical indicators and quantitative analysis of gene expression results,to explore the dynamic changes in indoor reactive oxygen species and antioxidant system physiological indicators of rapid identification of low temperature wheat and interstitial and reactive oxygen content regulation related to the removal of the corresponding genes,in order to further study the genetic basis of wheat cold and genetic improvement potential lay the foundation.The results of this test are as follows:1.The content of H2O2 in the tiller stage and the content of malondialdehyde(MDA)in the 30 days of freezing and thawing were used to identify the cold resistance of the wheat cultivar.2.Compared with SOD and POD,the activity of APX and CAT in the tiller nodes of wheat cultivars was more favorable for improving the cold tolerance of wheat after 30 days of freezing.3.The trend of H2O2 content in frozen and delayed frozen wheat was similar to that in exposed winter.In CAT activity,the delayed freezing test was in good agreement with the field test results.4.The expression of Ta SOD-3 gene in winter wheat(30 days and 10 ? 2 h)was the highest in Dongnong Dongmai 1,which was 8.63 and 11.78 times higher than that of Chinese spring.5.The expression levels of Ta APX-2 and Ta APX-3 in Dongnong Dongmai 1 were the highest in China during the freezing period(-12 2h),?which was 3.12 and 21.22 times higher than that of Chinese spring.6.TaSOD-3,TaAPX-2,TaAPX-3 genes are important genes involved in low temperature response.Among them,Ta SOD-3 gene plays a major role in the early cold stress,and Ta APX-2 and Ta APX-3 play a major role in the continuous freezing.