The Effect Of Wolbachia On The Mating And Parasitic Behaviors Of Trichogramma Dendrolimi

Wolbachia is known as the most widely distributed and largest symbiotic bacteria group currently,which transmits by maternal inheritance.Wolbachia can participate in a variety of manipulations of reproductive activity of its hosts,including inducing the host cytoplasmic incompatibility,parthenogenesis,feminization of genetic male,male-killing and improving female fecundity and male fertility.It is thought to be closely associated with some important biological problems,such as sex determination and speciation.And it is well-known that the egg parasitoid wasps of Hymenoptera can cause the death of its host eggs,by means of parasiting the host eggs.This research progress could play an essential role in the biological control of crop pests in the fields.Therefore,in recent years,more and more researchers contribute to explore the reproductive and physiological effects of Wolbachia on egg parasitoid wasps,but there is few study involved the behavioral effects of Wolbachia on them.This study is on the basis of existing researches,thus,explores the influences of Wolbachia on mating and parasitic behavior of its host,Trichogramma dendrolimi,the specific contents and results are as follows:1.With the mating competition test of females of two strains and males of uninfected strain,male flap of wings were used to measure female attraction,and the first choice of male mating would be recorded.It was found that the mating experiment of Wolbachia-infected strain females in competition with uninfected strain females showed weaker female attraction,so that its courtship rates was down,and the courtship proportion of two strains in the test showed that Wolbachia-infected strain females was 53.33%,and uninfected strain females was 96.67%,respectively.In addition,males could court to both two strains of females in the mating compete experiment indeed,but they only chosen uninfected strain females in the end.2.Counted and calculated the female parasitism rate,ovipositor insert frequency and eclosion rate of two strains of females.It was found that there was no significant difference of the two strains in parasitism rate,with Wolbachia-infected strain of 22.90 ± 1.33%and uninfected strain of 25.47 ± 1.20%,respectively.Wolbachia-infected strain females showed a lower ovipositor insert frequency than uninfected strain significantly,two strains of females'ovipositor insert frequencies of Wolbachia strain was 15.80 ± 0.66 times/h and uninfected strain was 23.73 ± 1.74 times/h,respectively.But offspring eclosion rates of Wolbachia-infected strain females were significantly higher than that of uninfected strain females,and the offspring eclosion rates of Wolbachia-infected strain females and uninfected strain females were 95.60 ± 0.71%and 80.22 ± 1.8%,respectively.3.Observe and record the ovipositing behavior patterns sequence,the durations of the3.Observe and record the ovipositing behavior patterns sequence,the durations of the behavior patterns and the numbers of visited eggs,ovipositing eggs,female offspring eclosion,eclosion rate,fed on eggs and superparasitism eggs of two strains of females on the two different quality host eggs.The results showed that the ovipositing behavior patterns sequences of the two strains of females in fresh,high quality host egg laying eggs were the same and all quantitative indexes were higher than 20d low quality,storage eggs.The durations of each ovipositing behavior pattern in Wolbachia-infected strain females were almost longer than uninfected strain,but Wolbachia-infected strain had few differences in the durations of the behavior patterns and the quantitative indexes on both quality host eggs.The aim of this study is to provide theoretical basis for whether Wolbachia can get practical application in biological control,and to screen more high-quality natural enemy insects for lepidoptera larvae in the biological control of crop pests.