Effects Of PLIN1 On Lipid Metabolism And Inflammatory Cytokine Synthesis In Dairy Cows

Perinatal period is the high incidence of energy metabolism disorders such as dairy cow ketone disease and fatty liver.Due to the decrease of dry matter intake of dairy cow and the increase of energy demand,the energy is negative balance,which leads to massive mobilization of fat,causing ketosis,fatty liver and other energy Metabolic disorders.So the negative balance of energy caused by fat mobilization is the dairy cow perinatal energy metabolic disorders an important part of the disease.In addition,adipose tissue as an important endocrine organ of the body,secrete a variety of cytokines such as tumor necrosis factor(TNF?),interleukin 1?(IL-1?)and interleukin 6(IL-6),and these cytokines and ketosis and fat Liver and other metabolic disorders and mastitis and endometritis and other infectious diseases are closely related to the occurrence and development.PLIN1 is an opioid-coated protein that is expressed extensively in adipocytes and plays a key role in lipid metabolism.It is clear that the lipid metabolism of adipose tissue in dairy cows,especially the changes in the expression and activity of lipid metabolism enzymes,is the key to alleviate the fat mobilization and prevent perinatal energy metabolic disorders.At the same time,PLIN1 secretion of adipocyte inflammatory factors may also have a special role.Therefore,this topic intends to through the perinatal experimental cattle and in vitro culture of adipocytes and silencing PLIN1 in vitro experiments,in order to reveal PLIN1 adipose tissue lipid metabolism regulation mechanism and the mechanism of inflammatory response to the use of remission of fat Mobilize the control of dairy cows perinatal energy negative balance disease lay the theoretical basis.In vitro isolation of dairy cows primary adipocytes,transfection of PLIN1 overexpressing adenovirus and silencing si RNA.The results showed that PLIN1 could up-regulate the expression of SREBP-1c m RNA and protein and increase the expression of ACC,FAS,SCD and DGAT1 and DGAT2 in the downstream fatty acid synthase,and down-regulate the expression of PPAR? m RNA and protein,and inhibit the key enzymes of lipolysis HSL and ATGL And the expression of SREBP-1c m RNA and protein were down-regulated and the expression of ACC,FAS,SCD and DGAT1 and DGAT2 in the downstream fatty acid synthesis were significantly decreased by silencing PLIN1,While upregulating the expression of PPAR? m RNA and protein,and promote the expression of HSL and ATGL,which are key enzymes in lipolysis,leading to the decrease of lipid consumption and lipid droplets in adipocytes.In vitro isolation of dairy cows primary adipocytes,transfection of PLIN1 overexpressing adenovirus and silencing si RNA and addition of LPS stimulation.The results showed that overexpression of PLIN1 could inhibit the activation of NF-?B signaling pathway and decrease the expression of inflammatory factors such as TNF?,IL-1? and IL-6,and play an antiinflammatory effect.After silencing PLIN1,NFActivation of the signal pathway increased,TNF?,IL-1? and IL-6 and other inflammatory factors significantly increased the expression of inflammatory response to the occurrence and development.The results of in vitro experiments showed that PLIN1 promoted the accumulation of fat cells and the increase of lipid droplets,and could inhibit the inflammatory response caused by NF-?B inflammation signal pathway.