Potato Blackleg Disease To Plant Potatoes Pathogen Detection Technology Research

Occurred from sick potato potato black shank on potato black shank purified bacteria,according to Koch’s postulation certain pathogens,and extracted the DNA of the bacteria after using specific primers Ecalg and Eca2g for PCR amplification,DNA sequencing,eventually identified as Ereinia potato black shank subspecies(Erwinia carotovora subsp.atroseptica).Different concentrations of black shank pathogens acupuncture vaccination in the potato navel,observe the incidence,and the traditional method and improved method respectively to extract black shank bacteria DNA in the onset of organization,using the specific primer Ecalg Eca2g and PCR amplification to check whether this kind of potato with black shank pathogens.The results are as follows:(1)PCR was amplified by PCR using the specific primers Ecalg and Eca2g,and the size of the fragment was about 750bp.(2)When tlhe concentration of the inoculation was less than 9 × 105cfu/ml,the potato condition was the same as the comparison,without the disease,the potato with the black shin virus but not the disease;When the concentration was 1.8 × 106cfu/ml,the onset of the potato was developed.When the concentration of the inoculation is greater than 1.8×106cfu/ml,potato potato crisps become ill and are visible to the naked eye.(3)The traditional method of extracting a species of potato black shin was only 106cfu/ml，and the improved method was able to extract the DNA sensitivity of the potato black shin bug to 105cfu/ml.The choice of the two,the best choice in daily production practice is to use the modified method to make the extraction of the DNA of the black shin bug,and the sensitivity of the test is higher.(4)The traditional method is to extract a variety of DNA from the black tibia virus,which is a higher purity than the modified method,and the resulting DNA concentration is greater than the conventional method.(5)PCR was performed using the specific primers Ecalg and Eca2g,and the size of the fragment was about 750bp.The DNA of the black shank was used as a template.Indicating that the use of PCR amplification technology,can accurately detect the potato seed potato latent black shank bacteria.The study shows that the modified method can be used to extract of potato black shank pathogens of DNA,and by using PCR amplification techniques for rapid detection of potato pathogen,for potato black shank germs potato pathogen detection provides basis and methods.