| Waterlogging stress is one of the most serious natural disasters that people face,which seriously restricts the growth of plants.As the third widely cultiv ated vegetables in China,cucumber is weak in root system and well-ventilated,and is vulnerable to waterlogging stress.In this study,cucumber ultrastructure was observed under waterlogging stress using electron microscopy,and the dif ference protein induced by waterlogging stress was studied by using iTRAQ te chnique to reveal the physiological mechanism of cucumber response to waterlo gging stress and the biological and molecular mechanism of waterlogging stress.The main results are as follows:1、The submergence had little effect on the mitochondrial structure of Zao er-N,When added the agar,the mitochondrial membrane structure disappeared,the internal structure and the inner crista also disappeared.The chloroplast was compressed and deformed after submergence,and the lamellar structure was lo osened,and the stress intensity increased and the lamellar structure became loo ser.The time difference between the two different stress intensity was not obvi ous at the early stage,and the difference was significant at the later stage of f looding.2、The number of differential proteins detected in waterlogging and agar a dded waterlogging were 97 and 87.The biological processes involved in the d etection of differential proteins were cellular process,response to stimulus(Stre ss response),the main molecular function is binding(binding),catalytic activity(catalytic activity).Carbohydrate transport and metabolic profile proteins increa se during agar added flooding.3、There were 44 differential proteins in waterlogging and agar added wat erlogging,34 functional proteins could be predicted,21 proteins could be up-re gulated or down-regulated in agar added waterlogging,and 13 up-regulated diff erential proteins involved in the emergency.4、The results of qRT-PCR showed that the expression levels of cysteine-li ke receptor protein kinase 26,UDP-glycosyltransferase 92A1 and respiratory bu rst oxidase homologous protein B were different in different treatment Time tre atment were significantly higher than the control,and in different stress intensit y are gradually increased.Indicating that waterlogging stress promoted the expr ession of these three proteins,which may be important proteins induced by wa terlogging stress in cucumber. |
