The Impact Of The Transgenic Antiviral Wheat Line With WYMV-Nib8 Gene To The Soil Ecosystem

Wheat is one of the principal cereals in the world,and its requirements are increasing rapidly.The wheat yellow mosaic virus(WYMV)disease is one of the main diseases in the middle and lower reaches of the Yangtze River Winter Wheat District.N12-1,which contains the WYMV-Nib8 gene by particle bombardment,shows good disease resistance to WYMV by many years of multipoint tests,and now has entered the stage of productive experiment.In this study,N12-1 and its recipient parent Yangmai 158 were used to study the impact of transgenic wheat with disease resistance on rhizosphere soil ecosystrm.In this paper,the amount of main microbe,diversity of microbial communities and enzyme activity in rhizosphere soil has been investigated between the transgenic wheats and its repector.Our results could provide the basic data and promote further development of transgenic disease-resistant wheat for the safety assessment of transgenic disease-resistant.N12-1 and Yangmai 158 were planted in Luhe experimental station for transgenic crop,Jiangsu Academy of Agricultural Sciences(Luhe),in October 2014.In this text,rhizosphere soil was sampled at its four growth stages(sowing,jointing,filling and mature).The main result of this research as follows:1.Establishing the Real-time qPCR detective system of Arbuscular mycorrhiza,Fusarium graminearum,Pseudomonas fluorescens and Polymyxa graminis,this method was used to analyze the amount of main microbe,such as Arbuscular mycorrhiza,Fusarium graminearum,Pseudomonas fluorescens and Polymyxa graminis in rhizospheric soil at different growth stages of transgenic wheat N12-1 and its receptor wheat.The result shows that eliminate the effect of moisture in the soil,there was no significant difference in the amount of Arbuscular mycorrhiza,Fusarium graminearum,Pseudomonas fluorescens and Polymyxa graminis between transgenic wheat N12-1 and the receptor wheat.The amount of the microorganism increased first,and then decreased after a period of time.2.Polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE)was used to analyze the microbial community in rhizospheric soil at different growth stages of the transgenic wheat N12-1 and its receptor wheat.The result shows that there has a few different bands and has no significant difference between the transgenic wheat N12-1 and its recipient parent yangmail58.Besides,bacterial diversity was significantly higher than fungi.There was no significant difference in the Shannon-wiener index,the Evenness index and microbial diversity and principal component analysis.3.The activities of urease,sucrose and dehydrogenase in rhizosphere soil were investigated by phenol sodium hypochlorite colorimetry,DNS and TTC,respectively.The results shows that there was no significant difference during the growth periods if eliminate the effect of moisture in the soil.And there was no significant differences were observed between N12-1 and the non-transgenic wheat at both Luhe and Yangzhou.In summary,the amount of main microbe(such as Arbuscular mycorrhiza,Fusarium graminearum,Pseudomonas fluorescens and Polymyxa graminis),diversity of microbial communities and enzyme activity has no significant difference between the transgenic wheat N12-1 and its receptor Yangmai 158.Therefore,the transgenic wheat N12-1 with resistance to wheat yellow mosaic virus has no significant effect on soil ecosystem according to our dates in this research.This study can provide basic data for the evaluation of the environmental safety of genetically modified wheat.It also provides theory evidence for the technical foundation of safety assessment of genetically modified wheat and its commercialization.