| The goose plague is an acute or subacute septicemia that causes 3 to 20 days old goslings and young muscovy ducks caused by Goose Parvovirus(GPV),with high transmission,high morbidity and mortality Characteristics,to give goose industry caused a significant loss.In order to study the prevalence of goose plague in Guangdong Province,from September 2015 to December 2016,42 cases of goose liver and intestinal tissue with suspected goose plague symptoms were collected from Guangdong Province.Seven GPVs were isolated QY1 and QY2,which were separated from Qingyuan City in northern Guangdong,and GZ1 and GZ2 separated from Guangzhou in the Pearl River Delta region.YJ1 and YJ2 were separated from Yangjiang City in western Guangdong.MM were separated from Maoming City in western Guangdong.The seven GPV genes were sequenced.Their sequences,genetic evolution and glycosylation sites were analyzed.The results are shown below:In the whole gene analysis,the full length of the seven GPVs was 5050 bp.And the homology between them was 99.8% ~ 99.9%.And an independent new branch was formed in the phylogenetic tree.The homology of seven GPVs and GenBank was 93.4% ~ 98.9%,which was 98.8% ~ 98.9% with that of Chongqing strain.The homology with the standard strain B was 98.2 % ~ 98.3%.Analysis of NS showed that NS1 and NS2 of 7 GPVs possessed 1884 bp and 456 bp,respectively,which could be translated into 628 and 152 amino acids,respectively.The homology of NS1 of 7 GPVs was 99.7% ~ 100%,while that of NS2 was 100%.NS1 only mutations in individual bases and cause changes in amino acids,whereas NS2 did not undergo mutations in bases.7 strains of GPV formed an independent new branch in NS1 phylogenetic tree,which was closest to Taiwan strain 06-0329,82-0321 and Chongqing strain RC16,which the homology was 99.1% ~ 99.7%.The homology was 99.0% ~ 99.2% which compared with standard strain B.In the NS2 phylogenetic tree,an independent new branch was formed,which was close to that of Taiwan strain strain SHFX1201 and Chongqing strain RC16.The homology was 99.1% ~ 99.6%.The homology with the standard strain B was 98.7%.There were 4 identical glycosylation sites in NS of 7 GPVs,150-152 NKT,225-227 NYS,360-362 NWT,433-435 NST,respectively.But GZ2 was more than the others by 330-332 NAT glycosylation point.VP1,VP2 and VP3 of 7 GPVs were 2199 bp,1764 bp and 1605 bp respectively,which could be translated into 733,588 and 535 amino acids,respectively.VP1,VP2 and VP3 were 99.6% ~ 100%,99.6% ~ 99.9% and 99.6% ~ 99.9%,respectively.7 strains of GPV formed an independent new branch in VP1 phylogenetic tree,which was closest to the Guangdong strain strain GDFSh and Chongqing strain RC16,with the same origin of 97.7% ~ 98.2%.The homology with the standard strain B was 97.4% ~ 97.5%.In the VP2 phylogenetic tree,an independent new branch was formed,which was 97.8% ~ 97.9% homologous to the standard strain B.In the VP3 phylogenetic tree,an independent new branch was formed with Jiangsu strain JSXZ-4 and Anhui strain AH-1.The homology was 98.8% ~ 99.8%.The homology with the standard strain B was 97.9% ~ 98.0%.The VP of 7 GPVs had 5 identical glycosylation sites,219-221 NAS,331-333 NLT,582-584 NTT,700-702 NFS,712-714 NET,respectively.Analysis of ITR showed that the ITRs of 7 GPVs were 416 bp long.And their homology was 99.8% ~ 100%.7 strains of GPV in the phylogenetic tree form an independent new branch,with the Chongqing strain RC16 and Taiwan strain 06-0329 genetic relationship,the homology of 97.0% ~ 98.9%;with the standard strain B homology of 88.8%.In this study,seven GPV gene sequences,genetic evolution and glycosylation sites isolated from Guangdong were analyzed to understand the evolution direction and variation degree of the goose pestivirus in Guangdong Province.It would provide data basis to the Guangdong goose plague prevention and control. |
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