The Effect Of Jujuboside On The Expression Of HSP70 And HSP90 In Heat Stressed Broiler’s Brain

Objective Through the effect of jujuboside on the expression of HSP70 and HSP90 in broiler’s brain,explore the mechanism of jujuboside on the anti-heat stress of heat stressed broilers.Methods The one-day-old AA broilers needed in the grouped feeding experiment provided by the local hatchery(normal temperature and heat stressed in 34℃,diets were added 0,1.0 mg/kg jujuboside),obtain the brain samples of 4-week-old and 6-week-old AA+ broiler.Adopt the method of Real-time PCR and Western Blot analysis of brain HSP70 and HSP90 expression,using SPSS statistical analysis software.revealed the effects of heat stress on the expression of HSP70 and HSP90 and jujuboside of itsadjustmentResults 1.Adding Jujubosidesin the diets result in the ADFI and ADGhas an increasing trendin3-4 week old broiler’s brain.2.Heat stress result in the ADFI and ADG were significantlydecreased,F:G significantly increased in 6 week oldbroiler’s brain(P<0.05).Compared with the heat stress,adding Jujubosidesin the diets could relieve the adverse effects of heat stress on ADFI,ADG and F:G,on the heat stress add jujubosidehas a better effect.3.In the feeding experiment,add Jujubosides changed the expression of HSP70,HSP90 m RNA and proteinin the 4 week old broiler’s brain.The males brain HSP70 and HSP90 m RNA level wereincreased(P<0.01)significantly.In hens brain HSP70 m RNA were significantly lowerand HSP90 m RNA were significantly increased(P<0.01).In males brain HSP70 and HSP90 protein were significantly lower(P<0.01),in hens brain the HSP70 proteinwere significantly lower(P<0.01),however there was no significant change in HSP90protein(P>0.05).4.Heat stress result in the HSP70,HSP90 m RNA expressionsignificantlylower(P<0.05 or P<0.01),however the expression of protein were significantly increased in 6 week old males and hens(P<0.01).Add Jujuboside increased the HSP70 and HSP90 m RNA in males and hens’ brain.Add Jujuboside on the heat stress,increasethe HSP70 and HSP90 protein expressionin the 6 week old rooster,reducedthe hens expression.Add Jujuboside before the heat stress,the HSP70 and HSP90 protein expression in the rooster were significantlyor extremely significantlylower(P<0.05 or P<0.01)..Add Jujuboside before and on the heat stress reduce the HSP70 proteinexpressionin male and female,however significantly or extremely significantly reducedthe HSP90 protein expressionin the hens(P<0.05 or P<0.01)..5.Correlation analysis showed that ADG and ADFI were negative correlation with the HSP70 protein expression in the male and female(r=-0.726,P=0.017 and r=-0.681,P=0.030).Conclusions 1.Heat stress could significantly reduced the growth performance of broilers,add jujuboside could improve it.2.Heat stress had changed the expression of HSP70 and HSP90 Jujuboside regulated the HSP70 and HSP90 in heat stressed broilers.ADG and ADFI were negative correlation with the HSP70 protein expression.Jujuboside could improve the growth performance of heat stressed broilers,and could regulate the HSP70 and HSP90 expression in brain.3.The best time of add Jujuboside regulation the HSP70 and HSP90 expression is before or before and on the heat stress.