Study On The Change Of Early Serum And Intestinal Immune Factors In The Small Intestine Of Kazak Sheep

Objective: Echinococcosis,also known as hydatid disease,it is parasitized by the larvae of Echinococcus granulosus(E.g)in various intermediate hosts(humans and herbivores such as cattle,sheep,etc.)of the liver,lungs and other organs developed into a cyst caused by a zoonotic disease.The most suitable intermediate host of Echinococcus granulosus is sheep.In western pastoral area of China,sheep feather disease has seriously affected the development of sheep industry and the quality of life of farmers and herdsmen.Echinococcus granulosus in the host after a long-term adaptation process,can be ingested from the host body nutrition and survival,and basically isolated from the external environment,with the ability to disturb or escape the host immune response,so the epididymal study of hydatid disease is still one of the current research hotspots at present.On the basis of previous studies,the immune factors of early serum and intestinal tract of the small intestine of Kazak sheep were analyzed,and the changes of serum and intestinal immune factors in sheep infected with Echinococcus granulosus were preliminarily regular,for the late diagnosis and treatment of hydatid disease to provide the basis.Method: In the experimental group,a total of 10000 mature eggs were taken orally by every Kazak sheep,a total of 6,and the control group(n = 1).The blood was collected from the jugular vein at 0h before feeding,and the blood was collected at 6h,8h and 10 h after feeding.Two rats in the experimental group were sacrificed at 6h,8h and 10 h after the challenge,and the sheep in the control group were sacrificed at8 h,the small intestine(including duodenum and jejunum,the middle jejunum,the posterior jejunum,the ileum)were fixed with paraformaldehyde,and the secretion and distribution of immune factors in the small intestine of the experimental group and the control group were analyzed by immunohistochemical method.Result:1?IgA and IL-4 in the experimental group were significantly higher than those in the control group(P<0.01),and significantly decreased at 10h(P<0.01).Ig M increased significantly at 6h(P<0.01)for 10h;Ig G was significantly increased at 6h,significantly decreased at 8h,and significantly increased at 10h(P<0.01).IL-2 and CCL8 were significantly increased at 6h and 8h,respectively(P<0.01).TNF-? increased significantly at 6h(P<0.01),and decreased to 0h at 10 h.2?Ig A,Ig G and Ig M were mainly distributed in the basal gland cells of the small intestine,and Ig M was also distributed in the gland and the lamina propria of the small intestine.IL-2,IL-18 and IFN-?mainly distributed in the small intestine of the interstitial lymphocytes.The expression of IL-2,IL-18,IFN-? and Ig M in the intestine of the experimental group was higher than that in the control group,Ig M was the most obvious in the jejunum and ileum.,In the middle of the jejunum and ileum from the intestinal gland cells to the intrinsic membrane secretion;Ig G in the jejunum and ileum from intestinal gland cells to the intrinsic membrane secretion is most obvious.Conclusion:The levels of Ig A,Ig M,Ig E,Ig G,IL-2 and IL-4 in the early sera of Kazak sheep were analyzed and the immunohistochemical method was used to analyze the secretion and distribution of immune factors in the small intestine.Suggesting that Ig M,IL-2 in the six hook larva infection Kazakhstan sheep early play an important immune function.