Toxicity Effects Of Total Alkaloids Of Peganum Harmala Against In Vitro Cultured Sf9 Cell And The Larvae Of Spodoptera Fugiprida

Objective : The plant alkaloids are valuable resources for agricultural and pharmaceutical active substances.Based on biological activity,structure-activity relationships and mechanism of insecticidal alkaloids,looking for a unique mechanism of action of lead compounds has made tremendous achievements.Mechanism of insecticides not only play a significant alkaloids in the study of pesticide toxicology,but also provide important theoretical basis for the development and application of new pesticides.Alkaloids with diverse and so unique insecticidal activity and action targets.Research insecticidal mechanism of alkaloid,has been alkaloid pesticide R & D bottlenecks and difficulties.This study investigated the insecticidal mechanism of total alkaloids of Peganum harmala(TAPH)against larvae of Spodoptera frugiperda to provide basis for the development and application of alkaloids as a new plant insecticide.Method:TAPH was isolated from mature seeds of P.harmala and its cytotoxicity(0,1,5,10,and 20 ?g/mL)against S.frugiperda Sf9 cells was detected using MTT method.The effect of 50 ?g/mL TAPH treatment of 10,30,50,70 and 90 min on absorption of Na+ and K+ by Sf9 cells was measured,and the effect of 50 ?g/mL TAPH treatment of 1,2,3,4,5 d on the absorption of glucose was determined.Finally,leaf disc method(leaves disc in 20 ?g/m L TAPH dipping for 3 s)and injecting test(2 ?g / head TAPH)were used to detect the effect on blood corpuscle of 4th-instar larvae of S.frugiperda.Result:After 24 h treatment with 20?g/m L and 10?g/mL TAPH,the inhibition rates were 83.25% and 62.04%,respectively.The LC50 to Sf9 cells after treatment of 8,16,24 and 48 h were 35.32,23.21,14.87 and 8.98 ?g/m L,respectively.After 10 min treatment with TAPH,the absorption of Na+ and K+ increased rapidly,and the absorption decreased after 30 min.After 3 d treatment,the absorption of glucose increased rapidly,while the absorption stopped after 4 days.Treated by leaf disc and injection methods,the cell amounts of the 4th instar larvae of S.frugiperda decreased significantly,and recovered gradually as time extended.Conclusion:The total alkaloids had obvious toxicity against Sf9 cells.The absorption of Na+,K+,and glucose and the number of blood corpuscle of 4th-instar larvae of S.frugiperda were significantly inhibited.