Differential Proteomics Analysis Of Roots Of Panax Notoginseng With Different Content Of Ginseng Saponins

Panax notoginseng(Burk.)F.H.Chen is a perennial medicinal plant of Panax genus,and triterpenoid saponins is the significant medicinal active ingredients from its roots.At present,little is known of about the details of ginsenoside biosynthesis in Panax notoginseng.In this study,we used proteomics technology to identificate a large-scale proteome data that from the samples of Panax notoginseng with different levels of saponins on the nanoLC-MS/MS platform.The main purpose of the study is to find some novel problems beased on regulation of ginsenoside biosynthesis.The main results are as follows:1.The high phase liquid chromatography(HPLC)was used to measure five main ginseng saponins(Rg1,Rb1,Re,Rd,R1)from the roots of Panax notoginseng.The average content was measured in 0.22%±0.03 to 6.07%±0.28,1.07%±0.06 to 8.58%±0.58,0 to 3.25%±0.23,1.27%±0.06 to 10.74%±0.66,0.32%±0.01 to 2.33%±0.26,respectively.The change fold between the highest content and the lowest is 27.5,8.01,3.25,8.45,7.28,respectively.We select the samples of ginseng saponins on highest and lowest level as the samples for nanoLC-MS/MS analysis.2.The nanoLC-MS/MS platform was applied to analyse differential expression of proteins.468777 mass spectrum graphs was identificated in this study,and only 140834 mass spectrum graphs has been identificated as 7578 proteins,The identification of rate spectrogram is 30%.These proteins refer to some important biological processes and stress responses.The ion binding was considered as protein main molecule function.These main proteins was located in the cell membrane.In the KEGG database,the most proteins was identificated in ribosome pathway,The second is the biosynthesis of amino acids and carbon metabolism.3.In the these protein,1.5 fold change was used as the difference threshold,and we obtains 248 protein due to different expression,Down expression has 155 proteins,and up expression has 93 proteins.These differential proteins involved in biological processes of metabolic processes,The catalytic activities was considered as molecule function of differential proteins.These differential proteins was located in the cell cytosol.These proteins were involved in 11 pathways,including terpenoid biosynthesis,ribosomes,pentose phosphate pathway,monoterpene biosynthesis,mineral absorption,GSH metabolism pathway,DNA repairation,photosynthetic carbon fixation,unsaturated fatty acid synthesis,the amino sugar and nucleotide metabolism.4.Among the identified proteins,23 proteins were considered as the synthase in ginsenoside biosynthesis pathway,40 cytochrome P450,50 UGTs and 87 transcription factors were identificated in total prteins.The 6 cytochrome P450,3 glycosyltransferase,10 plant defense reactive protein and 6 antioxidant enzymes were identificated in the differential proteins.The results of correlation analysis revealed that 20 proteins were likely to be associated with the ginsenoside biosynthesis,stress response and adaptation,including MCS,CMK,HDS,AACT,IDS,CYP450,CYP82H28,CYP450 93A1,CPY450 monooxygenase,UDP-glucose flavonoid 3-O-glucosyltransferase UGT3,LRR-RLK At1g56130,LRR-RLK At1g27190,cysteine receptor protein kinase 29,G-type lectin RLK At4g27290,G-type lectin RLK SD2-5,peroxidase and glutathione peroxidase.