Irradiation Mutagenesis Breeding Of Eustoma Grandiflorum With Tissue Culture Plantlets

Lisianthus(Eustoma grandiflorum)is a biennial Gentiana Kelon common ornamental plant,its rich color,smooth and straight stem,leaf green,strain state light chic,beautiful flower shape,chic,elegant and bright color,with purple,blue roses substitute,has a high ornamental value and economic value,is one of the potted and cut type and very popular in the international currently.Mutation breeding is a new breeding method,which can produce a variety of phenotypic variation materials,expand the mutation spectrum,produce chromosome aberration and gene mutation,increase the variation type and shorten the breeding period.In this study,Lisianthus is about 4cm high tissue culture plantlets,60 Co gamma ray irradiation acute radiation induced mutation,and using the method of tissue culture combined with radiation breeding research.The results are as follows:1.Effects of different radiation doses on the 4cm high tissue culture seedlings of Eustoma grandiflorum.The results showed that the half lethal dose was 103.63 Gy,and the appropriate dose was 90 Gy,which could induce more variation,and the mortality rate was controlled within a certain range.In 0~150Gy ray range,plant mortality increased with irradiation dose increasing,while the proliferation rate was negatively correlated with the mutation rate.2.With MS as the basic medium,different concentrations of 6-BA and NAA,as well as activated carbon were added at different culture stages.The result shows that the active carbon has a promoting effect on rooting,root length and the fine,hard texture;radiation induced Eustoma grandiflorum plantlets optimal proliferation medium was MS+6-BA 2.0mg/L+NAA0.5mg/L+C1.0g/L,the proliferation rate of up to 307.69%;the best rooting for 1/ 2MS+NAA 0.5mg/L+ C1.0g/L induced rooting rate of 100% medium,and the growth of normal.3.The radiation treatment materials had obvious changes in shape: the mutant plants showed the characteristics of dwarfing,shortening and thickening of the stems,thickening of the leaves,changing the thickness of the leaves,or weakening the leaves,distorting the Yellow deformity,slow growth and development.4.After the irradiation,the guard cells of leaves of plants were contracted,and the degree of stomatal opening was obviously reduced.There were significant differences in the number of stomata,stomatal length,width and stomatal density(P<0.05)between the normal plants and the variation plants.The results showed that radiation treatment had a great degree of damage to the plant.At the same time,in the process of cell division,we found a large number of abnormal chromosome behavior,such as lagging chromosomes,chromosome bridges.5.A large number of plants with large phenotypic variation were obtained,which laid the foundation for identification and breeding of new varieties.