Effect Of Weak Light And Salinity Stress On Physiological Characteristics Of Two Species Of Clematis

Clematisfusca and Clematis serratifoliaare perennial wild plants,flowering at period of7,9 months,and color is very unique.mainly distributed in the Changbei Mountain,Jilin Province,they are suitable for the introduction and cultivation in the north of the garden to increase the diversity of plants.But the two kinds of Clematis growth habits and adaptability has not been systematically studied.Therefore,two kinds of Clematis have studied the weak light and saline-alkali stress.In this study,three-year seedling of Clematisfusca and Clematis serratifolia were used as test materials to study their characteristics under different light intensity and saline-alkali stress conditions.From the morphological structure,physiological and biochemical characteristics,it is convenient to explore the influence of adversity conditions on two kinds of Clematis,which provides some theoretical basis for the introduction and cultivation of two kinds of Clematis.Research indicates:1.With the decrease of light intensity,the morphological structure of Clematisfusca and Clematis serratifolia changed with the same degree.The results showed that Clematis serratifolia height and stem diameter increased with the decrease of light intensity and increase treatment time.Clematisfuscaheight rising withthe decrease of light intensity,stem diameterincreasing rate increases first and then decreases with the decrease of light intensity.The leaf area of ??Clematis serratifolia increased with the decrease oflight intensity,and then increased with the increase of the treating time.Clematisfuscasingle leaf area is increased first and then smaller under same conditions.With the reducingm of the linght intensity,Clematisfusca leaf water is on the rise.The Clematis serratifolia relative water content of the leaves decreased with the increase of treatment time.In the 40% and 70%light intensity conditions,the Clematis serratifoliachlb content increased with the decrease of the light intensity,and there was no obvious upward trend under weak light.The Clematisfusca contents of chla,chlb and chl increased with the decrease of light intensityat the early stage(10~ 20d),and increased at the later stage(30 ~ 40 d).2.With the decrease of light intensity,the soluble protein content of Clematisfusca and Clematis serratifolia decreased gradually,the content of proline increased first and then decreased,and the activity of POD decreased gradually with the decrease of light intensity.Enzyme activity generally decreases with decreasing of light intensity.The content of MDA in Leaves of Clematis serratifolia increased first and then decreased with the decrease of light intensity,and MDA content in Leaves of Clematisfusca was increased with the decrease of light intensity.3.The net photosynthetic rate of Clematisfusca and Clematis serratifolia decreased gradually with the decrease of light intensity.While Clematisfusca of LSP,LCP and AQY decreased gradually with the decrease of light intensity.Clematis serratifolia LSP,LCP and AQY are increased first and then lower.The Clematisfusca frequencies of Pn,Tr and Gs were significantly lower in the daytime than in the contrast group,while Ci was higher.Under the different light intensity,The Clematis serratifolia values of Pn,Tr and Gs at 70% and 90%light intensity were significantly lower than in the contrast group at,therefre higher than in the40% light intensity.4.Under the condition of 100 mmol/L ~ 200 mmol/L saline-alkali stress,the normal growth and development of Clematisfusca and Clematis serratifolia were not affected greatly,only a small part of the leaves appeared in the upper part faded;300 mmol/L~400 mmol/L,most of the leaves of Clematis serratifolia were decolorized and yellowed,and Clematisfusca most of the leaves were yellow and gradually blackened,and some plants died.5.NaCl and NaHCO3 solution,the chlorophyll content of Clematisfusca and Clematis serratifolia decreased with the increase of salt concentration and treatmengt time,and the contents of malondialdehyde and proline were decreased with the concentration of salt solution and Increased coercion time.Theactivities of SOD and POD in Clematis serratifolia were increased with the time of treatment,and the activities of SOD and POD in Clematisfusca increased with the 100 mmol/L,200 mmol/L.At 300mmol/L,400 mmol/L treatment,the activities of SOD and POD increased with treatment time increased first and then decreased.