Isolation,Identification And Immunogenicity Characteristics Study Of Porcine Parvovirus In Henan Province

Porcine parvovirus is one of the main pathogens cause reproductive failure of bigs.It can cause stillbirths,deformities and mummified foetus and sick piglets,and it can also cause dermatitis symptoms of piglets,non-suppurative myocarditis and emaciation syndrome.PPV often infect with PRV,PCV,CSFV,PRRSV and that aggravates the damage degree of PPV.Because of widespread popularity and harm of the disease,the disease has been one hot spot of the pig disease.We isolated and identified 4 PPV local strains,and studied their biological characteristics,also the whole genome sequencing were finished.In order to detect farther,we studied the inactivated conditions,and immunogenicity were also experimented with guinea pigs.Polymerase chain reaction(PCR)was used to detect the DNA of suspected porcine parvovirus(PPV)pathologic samples from Henan Province,4 PPV strains were successfully isolated by inoculating the positive pathologic material.Biological characteristics of the virus were studied by observing the lesions of ST cell,detected TCID50,HA titer of the virus and other physiochemical characteristic test.Specific gene fragment was amplified by PCR and the sequence was analysed.Compared with other PPV strains,the homology of the little gene sequence is 100%.It was confirmed that the isolated virus was belonged to porcine parvovirus.4 pairs of primers were designed according the PPV sequences published on Genbank to amplify the whole genomes,and the whole genome was sequenced.The result showed that the genome length of PPV strains isolated in Henan Provence were all 4679 nt.These PPV strains had the closest genetic relationship With NADL-2 strains,and distant with SR-1 strain.The results on PPV inactivated conditions has shown that both 0.5%of BEI and 0.3%of formaldehyde can completely inactivated PPV under the condition of 32?for 24 hours.These PPV strains were purified and inactivated with 0.5%of BEI,and mixed with the white oil adjuvants.The inactivated PPV suspension were injected to each guinea pig in test group,and the antibody level reached 26 after 28 days,the negative control group contain no antibody.