Genetic Diversity Of Leaf Color Cline Leymus Chinensis Offspring Under Heterogeneous Habitats

There was divergence between Leymus chinensis populations in many traits,including the most obvious characteristics of the different leaf color phenotypes.And the leaf is an organ sensitive to environmental change and plasticity organ,its structural is the most featuresreflect the plant adapt to the environment.Different leaf color trait of Leymus chinensis populations in the natural grassland were stable.The same leaf of Leymus chinensis is consistent throughout the growing season in the same habitat.The naked eye can clearly distinguish the different leaves colour of Leymus chinensis from the typical gray-green to the typical yellow the gray-green between the green,several times yellow-green and intermediate leaves leaf colour,there is an obvious leaves cline.In this study,five kinds of leaves of L.chinensis seeds in different soil types the Changling.Tumuji.and Guyuan three natural grassland used as experimental materials to study the genetic diversity of the first Leymus chinensis offspring,offspring of Leymus chinensis leaf cline in the DNA levelgenetic structure and genetic diversity.The results show that 19 primers detected the amplification of locus 347,where the ratio of polymorphic loci of up to 100%.For different regions of Leymus chinensis populations the rate of polymorphism is 90.01%,for the different leaves of L.chinensis populations Leymus chinensis populations the rate of polymorphism is 68.21%,and these indicate that L.chinensis has a high genetic diversity.From the observed allele of Na,Ne of the effective allele of polymorphic loci,polymorphic loci ratio P and Nei's genetic diversity index,Shannon's information index to analyze the Leymus chinensis population genetic diversity the results were always decresing in in accordance with Changling and Tumuji and Guyuan,The Guyuan intermediate color of L.chinensis had the highest genetic diversity,while times yellow-green L.chinensis had lowest genetic diversity.Five leaves of L.chinensis populations in three regions,the gray-gree or sub-high leve L.chinensis had higher genetic diversity,yellow-green L.chinensis had lower genetic diversity.FigureTumuji the middle color or yellow green Leymnus chinensis population has high levels of genetic diversity,gray-green Leymus chinensis populations has low levels of genetic diversity.Guyuan region intermediate color Leymus chinensis population has high levels of low levels of genetic diversity.Ci yellow green Leymus chinensis population has low levels of genetic diversity.L.chinensis populations of different soil types in three regions of Na and Ne,polymorphic loci.P,H,the I were declined in accordance with Changling meadow Guyuan chestnut Tumuji chestnut>Changling salinization>longRidge sandstorm>Guyuan the salinization Tumuji salinization.Application AMOVA analysis studied genetic variation in Leymus chinensis populations in the three regions,three regions of different leaves and three different soil types.The three regional analysis between populations and genetic variation within populations were 13%and 87%respectively.Three regions of five leaves of L.chinensis and Changling of 14%and 86%.9%and 91%Tumuji.Guyuan region of 17%and 83%of genetic variation and populations andwithin populations.L.chinensis of different soil types in different regions and Changling of 30%and 70%.37%and 63%of the Tumuji Guyuan region of 42%and 58%of genetic variation and among populations and within populations.In summary Leymus chinensis populations in the genetic variation is greater than among populations,indicating that L.chinensis leaves cline of genetic variation within populations.The genetic identity was the highest and the genetic distance was the lowest of Leymus chinensis leaf cline in Changling and Tumuji region.It revealed that they had the closest relationship.The genetic identity was the lowest and the genetic distance was the highest of Leymus chinensis leaf cline in Changling and Guyuan region.It showed that they had the farthest relationship.The genetic identity was the highest and the genetic distance was the lowest of grayish-green and demitint leaf color Leymus chinensis population in Changling region.It indicated that they had the closest relationship.The genetic identity was the highest and the genetic distance was the lowest of yellowish-green and yellow-green leaf color Leymus chinensis population in Tumuji region.It revealed that they had the closest relationship.The genetic identity was the highest and the genetic distance was the lowest of grey-green and demitint leaf color Leymus chinensis population in Guyuan region.It revealed that they had the closest relationship.The five kind leave color of each soil type first clustered into a class and formed an evolutionary unit exception gray-green of Changling meadow.Gray-green or yellow-green leymus chinensis was always the first to differentiate in other soil types exceptin Changling saline-alkali soil.Used Network software to analyze leymus chinensis populations,saline-alkali soil was firstly differentiated,then the soil,finally meadow soil in Changling ares.Chestnut soil of Tumuji and Guyuan was firstly divided then the saline-alkali soil division.Leaf differention was mainly due to genetic drift caused the formation of the differention leaf color phenotypes in diffenent habitats:but the differentiation result was same and formed the differentiation different phenotypes in different soil types,climatic conditions and the pressure to suivive.the population has the same parallel evolutionary process.