| Landscape fragmentation was proved that it had important impact on biodiversity.Thousand-Island Lake(TIL)is typically fragmented landscape caused by impoundment.Our study used mitochondrial DNA COI gene fragmented sequence to explore the effect of landscape fragmentation to genetic diversity of Dendrolimus puncatus population gathered from 23 islands in TIL,results are as following: 141 distinct haplotypes were identified and the level of 23 populations was relative high.The value of haplotype diversity(Hd)ranged from 0.85375 to 0.98268.Tajima's D test indicated that D.punctatus population was not suffered bottleneck effect and orientation selection.Regression analysis between genetic diversity and island attribution showed significant positive correlation between genetic diversity of D.punctaus population and island area,as well as island shape,but no relationship between genetic diversity and isolation of islands(distance to nearest island).Pairwise Fst value ranged from 0.00025 to 0.18805,the level of differentiation between population was relative low.The proportion of high differentiation level was only 1.98% and medium differentiation level was 17.39% and the others were not differentiated.Regression analysis showed no correlation between genetic distance and geographic distance.The results of AMOVA showed when 23 populations were divided to 2 groups(I33 and remaining populations),there was significant genetic structure between the 2 groups.Variance within populations accounted for 89.71% of total variance and it was the main source of variance of D.punctatus population in TIL.Phylogenetic trees showed no genetic structure between each haplotypes. |
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